Preparation Of Three-dimensional Nanoporous Material And Its Application In Biosensors

Three-dimensional nano-porous material with conductive ability possesses continuou ligaments and pores.Its continuous conductive ligaments allow electrons to conduct continuously,which attribute to its good electrical conductivity.Their continuous pores allow for efficient diffusion of solute molecules,resulting in rapid material transport.Trace dopamine testing is crucial for the early diagnosis and prevention of certain diseases,such as Parkinson's disease,Alzheimer's disease and schizophrenia.In this study,a novel self-supporting three-dimensional bicontinuous porous gold?NPG?electrochemical biosensor was constructed and its dopamine?DA?detection performance was analyzed by differential pulse voltammetry.The palladium nanoparticles was deposited onto self-supporting nanoporous gold wires by electrochemical deposition.Due to the synergistic effect of the excellent catalytic activity of palladium and the novel structure of the NPG wire,the novel Pd nanoparticle-modified NPG wire biosensor has great advantages in the detection of DA.Its detection range:1-200??,sensitivity:0.43?A??^-1,detection limit:1??.Alpha-fetoprotein?AFP?,an important tumor marker,is widely used to diagnose patients with germ cell tumors and hepatocellular carcinoma.In this paper,a label-free,electrochemical immunosensor was developed using a composite of Pd/PPy conducting polymer hydrogels to detect AFP.On the one hand,the PPy conducting polymer hydrogels combine the advantages of organic conductors and hydrogels and possess an excellent conductivity and biocompatibility.On the another hand,the PPy conducting polymer hydrogels also possess a three-dimensional,bicontinuous,nanoporous,conducting network structure,allowing efficient mass transport.In addition,Pd has an outstanding catalytic activity and enhances the signal amplification.All of the above characteristics result in the excellent detection performance of PPy conducting polymer hydrogels for AFP.The detection range was 0.001 ng mL^-1to 300 ng mL^-1,and the sensitivity was 25.8 5?A(lgC_AFP)^-1.After30 days,the detection signal still retained 96%.Biochips can detect proteins,nucleic acids and other biomolecules accurately,quickly and with high throughput.However,bacterial interference can seriously affect its application in the field of detection.Therefore,it is of great significance to build biochips with antibacterial properties.In this study,UV-light initiated and polymerized on the substrate to form a patterned conductive Ag/PPy polymer hydrogel.The patterned conductive hydrogel not only has the advantages of good conductive properties,large specific surface area,good biocompatibility,and three-dimensional nano-porous structure,but also has good antibacterial properties due to the presence of Ag particles.It can not only have excellent detection performance on biomolecules,but also avoid the interference of bacteria and the influence of bacteria on the detection performance.