Study On Expression Of The Wnt And FGF Signaling Pathway Related Genes In Embryonic Development Of Brachyury Mutation Model Mice

Brachyury plays a significant role in the regulation of mouse embryonic development,especially the construction of mesoderm.Mouse embryos lacking Brachyury protein do not develop normally,in addition,Brachyury mutant mice exhibited short-tail phenotype.Wnt and FGF signaling pathways could control embryonic axial development,EMT and other physiological processes during mouse embryonic development,and Brachyury may cause the short-taile phenotype via the interaction with above two signaling pathways.To reveal the potential interactions between Brachyury and Wnt and FGF signaling pathways.We first observed the spatial expression of Brachyury during mouse embryonic development via the whole embryo in situ hybridization technique which used a convenient way to synthesize RNA probes.Meanwhile qPCR used to detect the expression of Brachyury,Wnt and FGF signaling pathway-related genes by extracting the total RNA of mouse embryos in the Brachyury mutation model mice at different developmental stages.The experiment results show that the expression of Brachyury gradually increased at 7.5 dpc in wild-type mouse embryos,peaked at 9.5 dpc,and then gradually decreased.Brachyury expression in mutant mouse embryos was consistent with that of wild-type mouse embryos,but the expression level was significantly lower than that of wild-type embryos at the same time.The expression level of Ainx2 in wild-type embryos was significantly higher than that of mutants;The expression trend of Dkk1 was similar to that of Brachyury and the expression peak of mutant embryos was higher than that of wild embryos;and the expression of Wnt3 was significantly higher than that of wild-type embryos since 8.5 dpc.Furthermore,the wild-type expression of Wnt3 a and FGF4 were significantly higher than the mutant at 9.5 dpc and formed expression peaks.Therefore,as a transcription factor,Brachyury regulates the expression of two signaling pathways components,and they form a certain feedback loop and regulatory network to regulate the development of mouse embryos.This study revealed the potential relationship between Brachyury and Wnt and FGF signaling pathway-related components,which provided a theoretical basis for the functional study of Brachyury(T)during mouse embryonic development.