Immobilization Of Shewanella Oneidensis MR-1 On An Anode Surface Improve Performance Of Sediment Microbial Fuel Cells

Anodic microbial community especially exoelectrogens is an important factor of sediment microbial fuel cell(SMFC)performance.In this study,Shewanella oneidensis MR-1 was immobilized on active carbon fiber anode and the SMFC's performance was evaluated.The SMFC with immobilized strain MR-1(SMFC-ImSW)displayed a maximum power density of61.0mW/m~2.The maximum power density of SMFC with conventional bioaugmentation of strain MR-1(SMFC-SW)and no strain MR-1treatment(SMFC-CK)obtained 11.1 mW/m~2 and 2.4mW/m~2 respectively.Furthermore,after 180 days experiment,the total organic content(TOC)and dissolve organic content(DOC)removal efficiencies of SMFC-ImSW were 59.91±2.03%and 43.85±1.15%,followed by SMFC-SW were 53.99±1.87%and 33.79±0.67%,SMFC-CK were abtained 44.07±1.15%and 16.47±3.46%respectively.During the wastewater treatment stage,the chemical oxygen demand(COD)removal efficiency of SMFC-ImSW was increased14.2%and 24.2%compared to SMFC-SW and SMFC-CK respectively.The GFP-tagging and RT-PCR results showed that strain MR-1 has colonized the anode biofilm of SMFC-ImSW.The microbial community structure analysis showed the relative abundance of bacteria related to electricity generation were increased significantly in anode biofilm of SMFC-ImSW.Therefore,immobilized bioaugmentation is a potential approach to increase voltage generation and purify contaminant sediment and water.