The Mechanism Of P-Stat3 Regulates The Social Behavior And Metabolism In The Nucleus Accumbens Of Brandt's Voles

Background Oxytocin(OXT)plays an important role in the central nervous system.Oxytocin not only can control social behavior of people and animals,but also regulate appetite and fat metabolism through the leptin induced signaling pathways.And then,oxytocin disorder can cause mental disorder and metabolic diseases.Oxytocin has been applied to mental illness,obesity and diabetes.Further studies of oxytocin signaling may reveal the mechanisms of these diseases.In view of the relationship between P-Stat3(Phosphorylation signal transduction and transcriptional activators)and leptin and oxytocin,we hypothesize that the P-Stat3 can regulate expression of oxytocin or oxytocin receptor(OXTR).And mental illness and obesity influence each other by the P-Stat3.Method In this study,3 months old Brandt's voles(about 50g)were selected,and P-Stat3 inhibitor was injected into the nucleus accumbens(NAcc).The changes of social behavior of Brandt' voles were observed by social recognition experiments and the central arena experiment.To verify the relationship of P-Stat3 and oxytocin further,we microinjected oxytocin,oxytocin receptor inhibitor,mixture of oxytocin and P-Stat3 inhibitor into NAcc respectively.The expression of oxytocin and oxytocin receptor was tested by Western blot.The metabolic cage test was used to detect the changes of basic metabolism of Brandt's voles and weight was recorded regularly.Results When P-Stat3 inhibitors were injected into the nucleus accumbens of Brandt's vole,the social recognition ability decreased and the attack behavior increased.The expression of oxytocin decreased,however the expression of oxytocin receptor had no significant change.The expression of leptin decreased significantly in NAcc.Meantime,the basal metabolism decreased and the weight increased significantly.Conclution Social behavior and obesity of Brandt's voles were regulated by the nucleus accumbens and influenced by P-Stat3 inhibitors.And mental illness and obesity influence each other by the P-Stat3.