Study On Purification Technology Of Black Odorous Water By Immobilized Microorganisms

The existence of black odorous water bodies greatly inconveniences the life and health of the surrounding urban residents,and at the same time affects the overall beauty of the city,and will lead to a large number of deaths of aquatic animals and plants in the water,threatening the ecological balance of water.At present,the main methods of governance include physical methods,chemical methods,and bio-ecological methods.The bio-ecological method is the safest and environmentally-friendly treatment method that is most closely linked to the construction of sponge cities.At the same time,there are few studies on the use of indigenous microbial immobilization to purify black odorous water bodies.The screening of indigenous microbial immobilization has broad application prospects and provides reference for the future purification of black and odorous water bodies.Therefore,this paper selects microbial immobilization technology to study the black odorous water body,and takes the black odorous water body as the research object,screens the immobilized subatrate and the dominant indigenous microorganisms,and uses the immobilized microbial technology to achieve the purpose of purifying the water body.The research results are as follows:The black odorous water body in Jiancheng District affects the living and health of the surrounding residents,destroys the harmony of the urban landscape,and at the same time causes a large number of deaths of aquatic plants and animals in the water,thereby threatening the water ecological balance.At present,the treatment of black odorous water mainly includes physical method,chemical method and bio-ecological method.The bio-ecological method is a safety and environmental protection measure that is consistent with the construction of sponge city.At present,there are few reports on the use of immobilized indigenous microorganisms to purify black odorous water bodies.Therefore,this paper uses black odorous water bodies as research objects to screen immobilized substrates and dominant indigenous microorganisms,and uses immobilized microbial technology to purify water bodies in order to treat black odorous water bodies in the future.Providing technical reference.The main conclusions are as follows:?1?Screening of immobilized substrates.There are 8 subatrates include red zeolite?HF?,green zeolite?LF?,ceramsite?TL?,corncob?YM?,quartz sand?SY?,volcanic stone?HS?,vermiculite?ZS?and peat soil+vermiculite?NTZ?.Eight kinds of substrates were used to investigate the effect of subatrates'size,addition amount and adsorption time on the purification effect.The results showed that when the addition amount was 8%and the adsorption time was 150 min,the removal rate of NH₃-N,TP and COD was the highest in LF with small particle size,which were 80.31%,56.31%and 47.91%respectively.LF is used as an immobilized microbial carrier subatrate.?2?Screening for dominant indigenous microorganisms.A total of 14 indigenous microorganisms,including 11 strains of bacteria and 3 strains of yeast,were obtained after in-situ black odorous water bodies were purified by culture and separation.By examining the effects of microbial inoculum,pH and ambient temperature on microbial degradation,a strain of bacteria B2#was finally selected.The results showed that the degradation rate of B2#was better than that of other strains.When the inoculation amount was 3%,the pH was5.5-6.5,25°C,the degradation rates of COD,NH₃-N and TP in black odorous water bodies were 36.51%and 40.21%and 45.71%,respectively.?3?The above-mentioned immobilized subatrate LF and microbe B2#were adsorbed and immobilized.The results showed that the removal effect of immobilized microorganisms on COD,TP and NH₃-N was significantly better than that of single free microorganisms or subatrate.The degradation efficiency was 49.51%,70.01%and 71.11%;by analyzing the influencing factors,optimizing the treatment effect,finally determining the dosage of immobilized microorganisms to be 5g/100ml black odor water,the rotation condition is 120-150r/min,the ambient temperature is 25°C,and the degradation period is24h,the microbial degradation was considered to be more rigorous,and other expressions were similar.The removal rates of COD,TP and NH₃-N were 53.41%,71.91%and 70.61%,respectively.