Sensitive Discrimination Of SNV Based On CHA Amplification-competition Inhibition Strategy On The Surface Of Microtube

In this work,we use multiple engineering network to significantly improve the effect of hybridization probes in selectively recognizing single nucleotide variants(SNVs).We built a detection system that catalytic hairpin assembly(CHA)amplification work in parallel with a competitive inhibition system.The amplification probes bind with correct target perfectly while the competitive probes mostly bind with mutant target after special designed.Then we combined the multiple detection system with polydiacetylene(PDA)microtube optical waveguide platform as a biosensor to discriminate different members of let-7 miRNA family that only differ in one single base position.The result showed that discrimination factor(DF)increased to 15 which are almost 10-fold compared to single amplification system.Besides we applied proposed multiple biosensor to detect human serum and discriminate the let-7 members in serum successfully.This work innovatively created a novel detection system which combined CHA amplification strategy with similar competitive inhibitor strategy and discriminated SNVs at an extremely low concentration(15fM).We provide an unique route for both academic research and clinical practice.Besides we also study the influence of chirality and topological-structure on the cytotoxicity of homopolymer vesicles.Herein,we severally synthesized linear polymers(LNPs)and hyperbranched polymers(HBPs)with L,D and racemic types and study the enantioselective cytotoxicity of optically active vesicles.Compared with HBPs vesicles,LNPs vesicles have properties of higher surface charge density and more violent interaction with simulated biomembranes which result in larger cytotoxicity against HeLa cells.Specifically,racemic-LNPs vesicles exhibit the largest cytotoxicity of all.More interestingly,there is no significant enantioselective dependence of HBPs vesicles in the abovementioned properties.In summary,this work provides new insights into vesicles inducing cancer cellular injury by demonstrating autophagy associated with the chirality and topological-structure and provide a novel for designing bio-interface materials for cancer cell therapy.