The Mechanism Of RBM47 Phosphorylation In Cardiac Regeneration

Cardiac regeneration is a complex and sophisticated process involving cell proliferation,differentiation and tissue morphogenesis,and RNA-binding proteins(RBPs)play a key role in this process.RBM47 is an RNA-binding protein that regulates alternative splicing,base editing and polyadenylation of mRNA,and has significant functions in early neurodevelopment and tumor growth.We found that the expression level of RBM47 is enhanced in neonatal and adult zebrafish with cardiac injury and can form dimers and spliceosome.We identified multiple phosphorylation sites in RBM47 and demonstrated that the formation of RBM47 dimers is associated with the post-translational modifications.Cardiac injury activates phosphorylation of S309 in the RNA-binding domain of Rbm47 and inhibits phosphorylation of T314,suggesting that phosphorylation of RBM47 S309 may be involved in cardiac regeneration.We identified GRK2 as a phosphokinase of Rbm47 S309,which may mediate the response of RBM47 to external stress.Phosphorylation of RBM47 S309 and T314 can be activated by hypoxia(1% O2),and mental stress can also inhibit their phosphorylation to some extent.In summary,our study focused on RBM47 phosphorylation,discussed how RBM47 phosphorylation is linked to its dimerization and splicing,explored the role of protein kinase-mediated RBM47 phosphorylation in cardiac regeneration.