Synthesis And Characterization Of Hypochlorite Fluorescent Probe With Acetal Ammonia Unit As Recognition Group

Reactive oxygen species(ROS),which refers to oxygen containing molecules with high reactivity to biomolecules,play important roles in cell signaling and homeostasis including oxidative damage,aging,anti-inflammation regulation and pathogen response.Among various ROS,hypochlorous acid(HClO)or hypochlorite(CIO"),as an endogenous microbicidal agent with highly potent oxidant which is mainly generated by the catalysis of myeloperoxidase(MPO)in the presence of chloride and hydrogen peroxide under physiological conditions,plays pivotal roles in human immune-defense system for defending and killing pathogens.But,If excessive hypochlorite is produced in human physiological activity,it would leads to the oxidation of biomolecules which results HClO was also used as a disinfectant in drinking water and swimming pools,and leads to many diseases,such as tissue injury,atherosclerosis,arthritis,neurodegeneration,lung injury and nephropathy.Therefore,sensitive and accurate hypochlorite detection probes are urgently needed in biological and environmental research.Fluorescent probes have long been considered as ideal candidates for environmental and biological studies because the detection process is non-sample-destructing and less cell-damaging.Fluorescent detection enjoys the advantages of simple instrumentation requirement,high selectivity and sensitivity,broad dynamic range and real time response,which make them especially suit for ROS detection in biological samples and processes.Recently reaction-based small-molecule fluorescent probes represented a promising technology for measuring biological species due to its sensitivity and convenience.So many efforts have been devoted to the development of fluorescent probe for measuring Hypochlorous acid and a number of organic small-molecule fluorescent probes for HOC1 sensing have been reported through modification of common fluorophores such as BODIPY,rhodamine,fluorescein,quinoline with HOCl/OCl-reactive groups.According to our research group designed and synthesis probe QBN,a dual-channal fluorescent probe detecting for HCIO and nitric oxiede.In view of outcome our predecessor have got,in the work,to improve the selectivity of probe we banned the exposed amino group,we chosed quinoline as an ideal component fluorescence chemosensor.Besides the fluorophore,the structural key points of the probe are the perimidine which can be oxidized by hypochlorous acid(HClO).Herin we designed and synthesized compound QDA.