Study Of Herpes Simplex Virus Type 2 Surface Glycoprotein B/D Novel Vaccines

Herpes simplex virus type 2(HSV-2)is one of the most common sexually transmitted diseases worldwide,which is neurotropic.Herpes simplex virus persists in the body through hiding in neuron cells.Genital herpes is a disease caused by herpes simplex virus type 2.It is usually transmitted by direct contact with the skin surface or secretions of infected persons.However,HSV-2 virus usually co-infects with HIV virus,which often increases the risk of HIV infection by 3-4 times.Genital herpes has the pHenomenon of one infection,lifelong latency,repeated recurrence,the decline of illumination,immune function,and pressure,all of which can cause the recurrence of the virus.At present,there is no effective vaccine to prevent or treat HSV-2 virus.After infected with HSV-2 virus,it can only be treated by taking antiviral drugs such as acyclovir.However,due to the asymptomatic exfoliation or mild itching often accompanied by virus infection,patients do not know that they have been infected,which makes the prevention of HSV-2 virus extremely difficult,and long-term use of antiviral drugs is also easy to cause to the production of tolerant drug-mutant virus strains,which makes the research of HSV-2 vaccine very important.Because of the recurrence of HSV-2 virus after infection,the vaccines of HSV-2 virus can be divided into two categories: preventive vaccines and therapeutic vaccines.Preventive vaccines are mainly used to prevent the infection of HSV-2 virus and the latency of HSV-2 virus with the animal model mice.While therapeutic vaccines are mainly used for patients infected with HSV-2 virus to alleviate the symptoms of virus recurrence and reduce the pain of patients with the animal model is guinea pigs.In this study,we studied the immunogenicity and protective effect of preventive and therapeutic vaccines against HSV-2 virus in mice and guinea pigs.(1)Two plasmids,Ucoe-mg B and Ucoe-gB-Ferritin,were constructed and their expression in 293 T eukaryotic cells was verified.(2)2936E cells were used to express two proteins in large quantities,and mice were immunized with DNA vaccine to evaluate the immunogenicity and the protective effect against lethal dose of mice.(3)Guinea pigs were immunized with half lethal dose of recombinant adenovirus vaccine rAd-gD,rAd-UL25 and rAd-gD-UL25 constructed in our laboratory to evaluate the protective effect of recombinant adenovirus vaccine on guinea pigs.The results showed that:(1)At the level of DNA vaccine,the introduction of Ferritin did not enhance the immunogenicity of gB.(2)DNA vaccine combined with DNA and protein vaccine had advantages in inducing cellular immunity and humoral immunity.Cellular immunity had a good scavenging effect on virus replication after primary infection in vivo,while humoral immunity had a killing effect on viruses of primary infection.Several vaccines induced Th1-biased Th cell immunity.(3)Recombinant adenovirus vaccine can induce specific antibodies and neutralizing antibodies in guinea pigs.The introduction of UL25 as an immunogen can protect guinea pigs better,reduce the frequency of virus recurrence,inhibit the occurrence of pathological conditions during virus recurrence,and to a certain extent,reduce the latent amount of virus in dorsal root ganglion.