Static And Dynamic GWAS To Explore The Genetic Mechanism Of The Interaction Between Escherichia Coli And Staphylococcus Aureus

In nature,the interaction between species is meaningful.The interaction between microbes also make a great contribution to maintain the balance and stability in the ecosystem.The general study of species interactions employed traditional methods of pure culture in a single species.This method ignored that the species survival is influenced by biotic and abiotic living environment factors,which can describe the effects of some genes on a single species.However,it is difficult to explain the underlying genetic mechanisms of interactions between multiple species,and it is rare to locate significant genes that affect interspecific interactions at the genome levelGenome-wide association analysis(GWAS)is able to search for genetic variants of complex shapes at the genome-wide level.This paper took the GWAS as a core.Utilizing two derived analytical methods:static GWAS and dynamic GWAS to correlate the growth traits and single nucleotide polymorphisms(SNPs)of E.coli and S.aureus.By gene annotation and functional analyze,ftsK,ftsZ,secA,trpA,thrA,rpoC,and yjjW were mapped in a static genome-wide association analysis.These genes have been verified to be involved in bacterial growth.In the dynamic genome-wide association analysis,the bacteria were analyzed in different culture conditions by combining with two dynamic analysis methods.The significant sites under different growth parameters were obtained.The candidate gene analysis was carried out and the interaction environment was found.Structural genes may affect different growth stages of bacteria,such asfepE,modA,carB,etc.In addition,thrA,ftsK,secA and yjjW genes were appeared in multiple times in both two analytical methods.It was initially assumed that these four genes had a more pronounced effect in the process of bacterial interaction.The discovery of these genes not only proves the accuracy of two analytical methods in this study,but also provides theoretical support for subsequent functional verification.In this paper,we used the method of genome-wide association analysis to link the phenotype of a species with a genotype.Finding trait-related loci and locating functional genes to explain the genetic and evolutionary mechanisms of microbial interactions in the genome-wide genome.It provides a theoretical basis for the study of bacterial genetic mechanisms.