Study On Microbial Diversity And Metagenome Of Ophiocordvceps Bispora Under Different Habitats

Ophiocordyceps bispora is a new record species in Asia.There are few studies about O.bispora at inland and abroad.In this study,high-throughput sequencing technology was used to analyze the strains of O.bispora in five different habitats through metagenomic research.The main results were as follows:In order to compare the microbial diversity in five different habitats,the mature O.bispora in five different habitats in Ailao Mountain were collected for amplicon analysis,through a,P-diversity analyze the similarities and differences of O.bispora in different habitats.The results of the petal plot showed that the unique OTUs in Ob.alD and Ob.alC were the most stable,and the stability was higher than other origins;UPGMA clustering trees clustered the samples,and Ob.alD was the most different among other groups in fungi and bacteria.Pseudomonas has the most dominant species and the species richness of Ob.alA is the highest;and the analysis of the dominant fungal flora shows that many Cordyceps parasitic has been noted that this discovery has great research value.In order to compare the differences between the functional genes of O.bispora in five different habitats,the amplicon analysis was further studied.Based on the macrogene technique,the species composition of the Ob.alA and Ob.alB groups was similar at the gate level.The difference is the biggest difference between with Ob.alD and both the Ob.alA and Ob.alB habitats are grassland,indicating that such a result has a great relationship with its habitat.The largest difference in the KEGG metabolic pathway was Ob.alA and Ob.alD.The biosynthesis of amino acids was up-regulated,and the amplicon results showed that the species diversity and community richness of the Ob.alA group were higher than those of the Ob.alD group.That the carbohydrate metabolism and amino acid metabolism of Ob.alA group are higher than Ob.alD group,which is related to the high abundance and diversity index of bacteria in Ob.alA group;the habitat of Ob.alA group is complex,and the habitat of Ob.alD is simple.Therefore,Ob.alA has a great relationship with the synthesis of glutamate-related glycine in the self-protection reaction in order to better cope with stress adaptation to the environment.The results of the resistance gene annotation showed that the abundance of Ob.alC and Ob.alD was lower than that of the other three groups,and Ob.alD lacked the tetracycline resistance gene family(Tet)compared with the other four groups.TetX.According to the literature,the concentration of resistance genes in the environment far away from the population is much lower than the concentration of resistance genes in the environment interfered by human activities,while the habitats of the Ob.alA and Ob.alB groups are close to the farmland and often have villagers.After the Ob.alC group is close to the top of the mountain and surrounded by steep mountains and densely populated trees;the Ob.alD group is the top of the mountain with fewer crowds;the Ob.alE group is a sparse coniferous forest,often with villagers grazing,Activities such as cutting trees and looking for fungi are close to the population,indicating that the causes of this phenomenon are related to habitats and crowd activities.This study analyzed the microbial diversity and metagenomics of O.bispora in different habitats,and identified the differences in microbes between O.bispora in different habitats.The multi-generation of fungal flora has great research value.At the same time,through the macro-gene research method,the differences of functional genes and resistance genes in the metabolic pathways of different habitats of O.bispora were obtained,which provided some direction for the study of its habitat and growth mechanism.