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Sequencing And Genetic Evolution Analysis Of BVDV-1 H2017 Strain

Posted on:2020-12-04Degree:MasterType:Thesis
Country:ChinaCandidate:X Y LiFull Text:PDF
GTID:2370330578452595Subject:Veterinary Medicine
Abstract/Summary:PDF Full Text Request
Bovine viral diarrhea virus?BVDV?is the causative agent of bovine viral diarrhea?BVD?,causing illness of cattle.It can show typical clinical symptoms,including diarrhea,mucosal erosion,mucosa ulcer,persistent infection,and so on.This disease has caused serious economic losses to the cattle industry in the world.The virus is divided into two genotypes,BVDV-1 and BVDV-2.It is also divided into two biotypes,cytopathic?CP?and non-cytopathic?NCP?.To understand the genomic information and genetic characteristics of the BVDV NCP H2017 isolate which was isolated and identified in our laboratory.In this study,according to 5 strains of BVDV-1 complete gene sequences published in GenBank,we designed 16 pairs of specific primers to the BVDV H2017 isolates gene.Determination of sequences by amplification by RT-PCR.Construction of genetic evolution tree by whole gene sequence,5'UTR sequence,Npro gene sequence and E2 gene sequence.The genetic characteristics of each part were analyzed to obtain the following results.This experiment completed sequencing of the BVDV-1 H2017 isolate gene,and the H2017 strain has a trusted gene of 12142 bp,G+C content is 45.97%,5'UTR is 303 bp,ORF is 11706 bp which from 3901 Amino Acid,3'UTR size is 133 bp.By aligning every homologies and constructing phylogenetic trees of the H2017 strain gene,5'UTR,Nprogene and E2 gene,it was found that the homology is 95.9%between H2017 strain and BVDV-1m LN-1 strain which is in the same branch of the genetic evolution tree;the homology between 5'UTR and BVDV-lm LN-1 is 95.3%;the highest homology is 99.2%between Npro gene and BVDV-lm NMG313 strain;E2 gene and BVDV-1m XC isolates are in the same branch of the evolutionary tree.According to the sequence comparison of NS2/3 gene between H2017 strain and BVDV-1 NADL strain?NC001461.1?,270 bp gap was found in NS2/3 gene of 4992-5263.The above test results show that BVDV H2017 isolate is BVDV-lm NCP virus.The test results not only enriched the molecular biological information of BVDV,but also enriched the epidemiological data of BVDV-1,providing reference for the in-depth study of the whole genome of H2017 strain and prevention and control of bovine viral diarrhea in Inner Mongolia.
Keywords/Search Tags:Bovine viral diarrhea virus, R T-PCR, Genome sequencing, Phylogenetic trees
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