Isolation And Identification Of BVDV And Proteomics Analysis Of BVDV-1 Strains Infected Cells

In this study,two BVDV positive samples were detected by RT-PCR from 32 bovine lung samples collected from a slaughterhouse in Hohhot,Inner Mongolia.They were identified by MDBK cells passage,immunofluorescence assay,5'-UTR sequence analysis and molecular evolution analysis.The results showed that there were no cytopathy after blind passage for 10 generations in MDBK cells;specific fluorescence was generated in the cytoplasm of virus-infected cells by IFA;phylogenetic analysis indicated that the strain belonged to BVDV-2a type.In successive studies we used DIA technology to quantitatively proteomic analysis of BVDV type 1 CP strain and NCP strain infected MDBK cells at 18hpi and 48hpi respectively.A cell control with uninfected virus was also provided.A total of 5094 proteins were quantified.Differential screening and bioinformatics analysis were performed on these proteins.Four groups of samples(CP 18 hpi,CP48 hpi,NCP 18 hpi and NCP48 hpi)were compared with the corresponding control groups,and 351,327,156,and 120 differential proteins were detected,respectively.Differentially expressed proteins are involved in biological processes such as metabolic process,biological regulation,and localization.There are differences in the differentially expressed proteins between the two biotype strains in the early and late stages of infection.This study provides a reference for the study of the pathogenic mechanism and prevention and control of BVDV.