The Mechanism Of Reversible Active Lysogeny In Bone Marrow Derived Macrophage For Prophage

This research was mainly on from a special phenomena termed reversible active-lysogeny in Listeria monocytogenes prophage?10403s.From the perspective of oxidation-reduction,studied the effect of redox on listeria virulence gene transcription,prophage?10403s excision,re-integration,and lytic cycle by oxidation and reduction in liquid medium,macrophage phagosome and cytoplasm.To draw a conclusion of the influence of oxidation and reduction to maintain balance among phage-intracellular bacteria–macrophage,the results were as follows:?1?Transcription of virulence genes was increased in BHI added with H₂O₂and phagosome,convincing that oxidation is one of the factors which induced increasing transcription of virulence genes.In BHI with low concentration of GSH and cytoplasm,transcription of virulence genes was also increased,which had the same effect with H₂O₂.However,high concentration of GSH reduced the transcription of virulence,that means the inhibitory effect of GSH on virulence genes transcription.All in all,breaking the balance of oxidation-reduction,which both impacted Listeria monocytogenes virulence transcription.?2?Oxidation induced the excision of prophage?10403s,as well as attracted phage to go into lytic cycle.?3?Exogenous GSH from macrophage or endogenous GSH synthetized by GshF in Listeria monocytogenes both inhibited phage lytic cycle in phagosome,but induced the re-integration of phage in cytoplasm.Therefore,oxidation would induce the transcription of virulence and excision of prophage,which increase the survival capacity of Listeria monocytogenes.At the same time,GSH would prevent the lytic cycle for phage,through that way,phage and bacteria could co-exist in macrophage.