Isolate And Identification Of Porcine Pseudorabies Virus Variation

Porcine pseudorabies is an infectious disease caused by pseudorabies virus?PRV?in pigs.In recent years,there had been reported virus variation of viral gene in this disease.In order to understanding the biological characteristics of the PRV wild-type strain in Sichuan,firstly,this study collected samples from the Sichuan'pig farms,and infected PK-15 cells to isolate a suspected pseudorabies virus strain?named as CHN-DJY2017?.Secondly,we amplified a gene fragment of about 288 bp by PCR;negative staining for TEM observed round virions with a diameter of about 100 nm;the infected rabbit showed itchy symptoms and acute death;the infected mice was showed acute pathology with obvious pathology change,;the serological neutralization test indicated that PRV positive serum can neutralizing this strain,and the TCID₅₀is as high as 10^-9.23/ml;all of this preliminarily proved that the isolate is PRV.Subsequently,we found that there were 7 consecutive amino acid sequences?AAASTPA?insertions at the 64th position of the gC gene,compared with the isolate sequences at home and abroad.It was also found that the genetic relationship between CHN-DJY2017 and foreign isolates is far by comparison of TK,gC,gE and gB genes.Finally,the biological characteristics of the isolate were identified.The strain had strong resistance to the outside,and had certain resistance to heat,ultraviolet,formaldehyde and so on.But it was sensitive to lipid solvents such as chloroform,and belongs to the enveloped virus.This study identified and analyzed the biological characteristics of PRV strains in Sichuan,which has great pathogenic significance for the prevention and control of pig pseudorabies in pig production.