Cloning And Function Analysis Of The Phosphate Transporter Genes In Thellungiella Salsuginea

Phosphorus is an important mineral nutrient for plant growth and development,and is a key component of biological molecules such as nucleic acids,phospholipids and ATP.Despite there are many forms of phosphorus in the soil,the availability of phosphorus is often limited and can not meet the needs of plants due to slow diffusion rate and uneven distribution.PHT1 gene family mainly regulates the uptake of phosphorus in soil by plant roots and the intercellular phosphorus transport in plants.Therefore,in order to improve phosphorus deficiency tolerance of plant,two genes were cloned from Thellungiella salsuginea which has a better tolerance to abiotic stress.Then they were transferred into plants to explore their functions and the transgenic lines with low phosphorus stress resistance were obtained.Based on the previous research,the expression patterns of 14 members of PHT1 family in Thellungiella salsuginea were analyzed by quantitative real-time PCR,and then Thhalv10003186 m and Thhalv10009424 m were cloned and named as TsPHT1;1and TsPHT1;8.The 35S:TsPHT1;8 transgenic Arabidopsis,35S:TsPHT1;1 transgenic Arabidopsis and soybean were obtained by Agrobacterium-mediated method.The35S:TsPHT1;8 Arabidopsis was treated with different concentrations of phosphorus,and the root length,lateral root density and phosphorus content were measured.Compared with the wild type plants,the root length and phosphorus content increased,and the lateral root density of transgenic plants decreased in the low phosphorus environment.The 35S:TsPHT1;1 transgenic Arabidopsis and soybean were cultured with Hoagland containing with different concentrations of phosphorus.The results showed that the growth performance of transgenic lines was better than that of the wild type plants under low phosphorus stress.Total phosphorus and inorganic phosphorus contents of the transgenic plants were also significantly increased under low phosphorus stress.The data indicated that TsPHT1;1 and TsPHT1;8 genes could improve the efficiency of phosphorus uptake and enhance tolerance to low phosphorus stress in transgenic plant.This study laid a foundation for the further insights into the mechanism of regulation of phosphorus uptake and utilization by plant PHT1 genes,and provided two potential candidate functional genes for obtaining transgenic plantswith high efficiency utilization of phosphorus.