Genome-wide Analysis Of Wheat Dehydrin And Function Insight Of WZY2 And Its Truncated Derivatives

Abiotic stresses can affect plant growth and even cause death.Dehydrin,a kind of highly hydrophilic,heat-stable,and intrinsically unstructured protein,plays a vital role in enhancing plant tolerance under abiotic stress.We have previously isolated the YnSKn type dehydrin WZY2,and demonstrated that it can protect the in vitro activity of LDH(Lactate dehydrogenase),and the overexpression of WZY2 in E.coli can also improve the relative activity rate during temperature stress.Here,we identify 49 wheat(Triticum aestivum)dehydrins and include some new dehydrins that were not studied in previous research.Motif analysis,chromosomal location,duplication event,phylogenetic relationships,gene structure analysis,subcellular location predictions were used to reveal the characteristics of dehydrin families;and overexpressed the WZY2 and Its Truncated Derivatives in Arabidopsis to explore its function.Taken together,these results indicate that these proteins are small molecule and hydrophilic dehydrin,most of them are YnSKn-type dehydrins(73.5%).All of the wheat dehydrin genes are located in the beginning of long arm and short arm of genomes,the gene structure of these dehydrins showed that almost all of the dehydrin genes contain one intron,moreover,the conservation of position and length of intros may also adaptive to the rapidly transcription when required.The phylogenetic tree of the subfamilies of wheat dehydrins showed close relationships and segmental duplication was the main expansion mechanism of wheat dehydrin genes.Those dehydrins displayed various expression pattern in different organs or under abiotic stresses.According to the organ-specific expression pattern,YnSKn-type dehydrin could highly up-regulated in embryo and seeding,indicated that YnSKn-type dehydrin play an important role in embryogenesis.dehydrins could up-regulate under drought,ABA and cold stresses,but had different tendencies.YnSKn type dehydrins are highly sensitive to drought and ABA treatment,but SKn and Kn type dehydrins were highly sensitive to cold conditions.overexpression of WZY2 and its truncated derivatives in Arabidopsis could improve the drought tolerance,and we speculate that WZY2 could enhance the stomatal closure through participant in ABA-induced stomatal closure signalling pathway and significantly improve drought tolerance of Arabidopsis.