Meat Quality Analysis Of The Crossbred Cattle With Myostatin Gene Editing

The inactivations of the myostatin gene in cattle often promote muscle development and improve meat production.To investigate the effect of MSTN artificial mutation on crossbreeding,carcass and meat quality traits of different parts in beef from cross-breed cattle of Luxi(MSTN^-/-)×Chinese Simmental were analyzed.The live weight and carcass weights of the two groups were 646.88±58.39kg,373.70±34.90kg and 584.41±54.76,314.40±29.04kg,respectively,as well as the dressing percentage of these cross-breeds reached to 57.76±0.01%,which was significant higher than Chinese Simmental?P<0.05?.Compared with the control group,the lightness and redness of Longissimus thoracis?LT?muscle from the MSTN mutated group were significantly higher?P<0.05?.There was no significant differences in yellowness?b*?,pH value,tenderness and water holding capacity between the two groups in the parts of high rib,ribeye,topside and shinleg?P>0.05?.The intramuscular fat contents in LT from the cross-breeds?5.90%?were significantly lower than that of the control cattle?6.74%??P<0.05?.The fatty acid composition was not significantly affected,with the exception of the polyunsaturated fatty acids?PUFA?proportion increased in the cross-breeds slightly.The gene expressions of the LT were detected by RNA-seq and varified by qPCR.It was found that the down-regulation of MSTN gene expression affected the expression of genes related to fat synthesis such as ADIPOQ,FABP4,FASN,LPL,PPAR?and SCD.It could be indicated that crossbreeding by MSTN artificial mutated cattle could increase the meat production,lead to insignificant effects on the most of meat quality.The decrease in intramuscular fat content of the LT muscle has a potential relationship with the inhibition of MSTN gene mutation and gene expression related to fat synthesis.