Separation And Identification Of GPV And Establishment Of Rapid Detection Method For LAMP

Goose parvovirus(GPV)is an acute or subacute septic infectious disease in goslings caused by Goose parvovirus(GPV).It mainly infects 4 to 20 days old goslings and has a very strong lethality,especially for the goslings of 10 days old.The main features of the disease are severe enteritis,shedding of the small intestine mucosa,necrosis and formation of characteristic intestinal embolism(acute type).Because of its rapid spread,high morbidity and mortality,the disease has brought serious harm to the breeding goose and has received much attention for many years.It is generally believed that the gosling can be made a preliminary diagnosis based on epidemiology,clinical features,and necropsy.However,the worst gosling plague,gosling flu,goose viral enteritis,and newly discovered goose paramyxovirus disease have similar clinical and necropsy features.To this end,it is of great significance to carry out laboratory diagnostic studies of gosling plover.In this study,the viruses from two parts of Changchun and pear trees in Jilin were isolated,and a 375 bp target fragment was amplified by PCR detection,which was consistent with the expected results.The products with positive PCR results were sequenced and analyzed.The sequencing results were compared with the GPV gene in GenBank.The results showed that the homology was up to 95% and it was confirmed as Goose parvovirus.And through animal regression tests,it was demonstrated that the isolated strain was a virulent strain.Since its development in 2000,the loop-mediated constant temperature nucleic acid amplification technology has achieved many achievements in diagnosis and detection,including clinical disease diagnosis,qualitative and quantitative detection of epidemic bacteria or viruses,and animal embryo sex identification.In this study,5 sets of primers were designed for the specific gene of GPV by using loop-mediated constant temperature nucleic acid amplification method.The GV-1 primer set was successfully selected as the best primer,and specific primers were used to demonstrate that the designed primer has good specificity.The selected optimal primer combination GV-1 was performed optimal temperature screening and the sensitivity experiments to determine the optimal reaction temperature 65?and the lowest detection template concentration 1.3pg/?L.