Study Of Stress Granules And Small Molecule Compound Favipiravir Against Human Enterovirus D68

Enteroviruse D-68(EV-D68),belongs to Picornaviridae.In 1962,it was found in children's nasopharynx swab in California.EV-D68 infection usually occurs in infants,particularly,people with a history of asthma are more likely to cause severe respiratory infections,pneumonia,bronchitis and other diseases.In the later stages of infection,it maybe fetal due to the complications of cardiopulmonary disease.There has been no effective vaccines and therapies recently.The research mainly discussed the novel immune response mechanism induced by EV-D68-infected cells,namely the mechanism of stress granules formation.And an novel antiviral drug called favipiravir.We have found that EV-D68 can induce the formation of stress granules which can inhibit the viral multiplication.SGs form during the early infection and inhibited at the late stage of infection.And then we found that it's EV-D68's non-structural protein called 2A protein lead to the phenomenons.2A protein can clevage SG's marker protein G3BP1,thus inhibit the formation of stress granules.In order to analyse SGs regulate EV-D68's proliferation,we designed some experiments to study the relationship between SGs and viral genome.And then we found that one of the components of stress granules TIA-1 can interact with the 5'UTR of EV-D68.The results can explain that TIA-1 can regulate the level of viral protein level through the 5'UTR.Favipiravir is a broad-spectrum molecule compound to RNA virus.When studing the antiviral drug,we determined safe concentration of the drug when used,and effective concentration for inhibiting the EV-D68's proliferation is 200? M.And we evaluted the antiviral activity of favipiravir through measuring the half effective inhibition concentration,compared with the other kinds of viruses,Favipiravir has stronger ability suppress the viral proliferation.It provides security in drug concentration selection during the next research.It is of clinical significance for the treatment of EV-D68.The conclusions in this study have enriched the understanding of the mechanism of EV-D68 infection and immune response mechanism pathway.More importantly,it is possible to prevent and treat the diseases induced by EV-D68.