Experimental Study On Isolation And Culture Of Newborn Rat Primary Hepatocytes

Objective:To establish a simple method for isolating and obtaining stable cultured neonatal rat hepatocytes.Methods:Primary rat HC was isolated by multi-step enzymatic digestion,and purified and cultured by low-speed centrifugation and selective adherence.Results:The number of separated HCs was about 1-2×10~6 cells per rat.After 0.4%trypan blue staining,the transient cell activity was>70%,and after cell suspensioncultured for 3 to 5 days,the cells spread 70%to 80%of the bottom of each bottle.The purity of the HCs was greater than 95%,and the cell growth were in good condition.Conclusion:The primary parenchymal cells of liver obtained were pure and in good activity,which laid the foundation for research on liver disease.