Study On The Differences Of Microbial Community Structure On The Surfaces Of Micro Nano Coatings With Different Antifouling Properties

The economic losses caused by marine biofouling are becoming more and more serious.The traditional antifouling coatings seriously damage the ecological balance of the ocean and cause serious environmental problems.Therefore,environmental friendly antifouling coatings have become one of the hot spots in the research of marine antifouling.The low surface energy composite antifouling coatings prepared by Carbon nanotubes and Polydimethylsiloxane have good antifouling properties,but there are few studies on the community structure and composition of the early biofilm on its surface damage.In order to study the mechanism of its antifouling performance,the structure of its biological community needs to be analyzed.Therefore,it is of great practical significance to study the structural composition and difference of biofilm on CNTs-PDMS.Three kinds of CNTs-PDMS coatings with better antifouling properties(Carbon nanotubes D12,D26 and D29)and three kinds of coatings with poor antifouling properties(Carbon nanotubes D32,D58 and D59).The 1mg/mL carbon nanotube material was add to the PDMS film system to prepare the CNTs-PDMS coatings.Through the ocean hanging plate,the early biofilm samples were collected for 3 days,8 days,15 days and 24 days,and the biomembrane genome was extracted.High-throughput sequencing of 16 S rDNA(V3+V4)and 18 S V4 regions resulted in sequencing data of prokaryotic and eukaryotic communities in biofilm samples.The results of eukaryotic high-throughput sequencing of 16 S and 18 S were analyzed,and the structure of biofilm community was analyzed by bioinformatics.In the early biofilm prokaryotic community,the dominant gates were Proteobacteria,Cyanobacteria and Gracilibacteria,and the dominant genera were Cycloclasticus,Unidentified Chloroplast and Alteromonas.In the eukaryotic community of early biofilm,the dominant gates were Annelida,Diatomea,and the dominant genera were Unidentified Spionida,Navicula and Unidentified Leptothecata.Through sample complexity analysis and multi sample comparison analysis,the diversity and abundance in the biological community of the CNTs-PDMS coating with better antifouling performance and the poor CNTs-PDMS coating showed obvious difference.At the same time point,the components in the biofilm sample group were relatively small,and the components in the biofilm sample group were very different at different sampling time points.With the increase of time,the similarity among groups decreased and the difference increased gradually.In many environmental factors,time and temperature are more associated with the CNTs-PDMS coated biofilm community,and the diameter,length,dispersion and antifouling of carbon nanotubes are less associated with the CNTs-PDMS coated biofilm community.The conclusion of this study is to preliminarily analyze the composition and differences of the prokaryotic and eukaryotic community structure of the early biofilm on the surface of the CNTs-PDMS coatings,which have distinct differences,and the environmental factors that have great influence on the biological community.The innovation points of this study are annotating the results of 16 S and 18 S high flux sequencing,the dominant gates and genera in the prokaryotic and eukaryotic biofilms of the CNTs-PDMS coatings are determined,and the species with distinct differences in different coatings are determined.The diversity and abundance of the coatings with better antifouling properties and the poor coatings are distinctly different.The results showed that different antifouling coatings could affect the species and content of biofilm community.The time and temperature were associated with the CNTs-PDMS coated biofilm community,and the diameter,length,dispersion and antifouling of carbon nanotubes were less associated with the CNTs-PDMS coated biofilm community.