Induced Fermentation Of Inonotus Obliquus And Its Effect On The Activity Of Triterpenes

Inonotus obliquus,because of its unique and magical physiological activities deeply loved by health and medical personnel.However,Inonotus obliquus is restricted by growth conditions and growth cycles and cannot meet human needs.Inonotus obliquus Triterpenoids is a natural active substance,which has many health care functions,such as anti-oxidation,bacteriostasis,anti-tumor,hypoglycemic and so on.In this study,Inonotus obliquus containing triterpenoids was used as the raw material,the method of combining inducer with liquid fermentation,the basic fermentation conditions were determined by single factor experiment,and then the optimum induction system was determined by optimizing the addition time of inducer and adding metrology to increase the content of triterpenoids.Single factor experiment combined with response surface method was used to optimize the technology of ultrasonic extraction of triterpenoids.Some activity tests such as antioxidant activity and antibacterial properties were carried out on triterpenes of Inonotus obliquus and triterpenes of natural Inonotus obliquus after liquid fermentation.The main conclusions of this experiment are as follows:1.The best basic liquid fermentation conditions for brown hole fungus were obtained through single factor tests:the fermentation cycle is 8 days,the rotation speed is 150 r/min,and the fermentation effect is best when the fermentation temperature is 26°C.The mycelium yield reaches1.018g/100m L The amount of triterpene of Inonotus obliquus was 48.86 mg/g.2.Under the condition that the inducer is contained,the brown hole fungus is obtained by liquid deep fermentation.The analysis results:?1?On the 6th day,methyl jasmonate added with ethanol co-solvent was significantly higher than?P<0.05?other groups.?2?The best conditions for SA induction were 100 mg/L and 0 d,and the total triterpenes were916.91 mg/L,which was an increase of 84.63%compared with the control group.?3?On the 6th day,1.5 g/L linoleic acid was added for induction fermentation.At that time,the total triterpene content was 775.58 mg/L,which was 56.28%higher than that of the control group.?4?On the 0th day of fermentation,adding 1 g/L birch bark water extract to obtain a triterpene compound peaked at 976.14 mg/L,which was an increase of 99.10%compared with the control group.?5?Early addition of Fe²⁺at a concentration of 6?M can maximize the amount of mycelium and further increase the production of triterpenes.The mycelium content reaches 10.44 mg/L,which is 2.04%higher than the control group.3.Optimized the conditions for triterpene extraction by response surface method.When the material-liquid ratio is 1:39,the ultrasonic time is 43.7 min,and the ultrasonic power is 362.5 w,the extraction rate is 3.78%.4.Quantitative analysis of Triterpenoids of Inonotus obliquus by high performance liquid chromatography showed that 50um of methyl jasmonate solution betulin,trametenolic acid and inodiol were added on the 6th day of fermentation.In 8 days,the maximum contents reached 14.68,2.03,and 2.55 mg/g,which increased by 115.25%,119.05%,and 93.18%over the same period without the addition of inducer.5.After the liquid fermentation of the brown hole fungus treated by the inducer,the triterpene of the brown hole fungus was extracted and tested for its biological activity.The analysis showed that:?1?IC₅₀ of triterpenes for DPPH·after induction by Me JA was 0.091 mg/m L,IC₅₀ of OH·was1.657 mg/m L,and IC₅₀ of·was 0.024 mg/m L.?2?Inhibition rate of hyaluronidase activity was 85.3%±1.28%when the triterpene concentration of Inonotus obliquus was 3 mg/m L.Up to 69.0%±0.93%.15%higher than normal fermented triterpenes?3?It was found that triterpenoids produced by induced fermentation were better than those of normal fermentation.The induced triterpene has the best antibacterial effect on Salmonella,and triterpenoids from normal fermentation had the weakest antibacterial effect against E.coli.