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Function Of Histone Variants H2A.Z In Fusarium Graminearum

Posted on:2021-02-01Degree:MasterType:Thesis
Country:ChinaCandidate:J H LiuFull Text:PDF
GTID:2370330602491911Subject:Biochemistry and Molecular Biology
Abstract/Summary:PDF Full Text Request
Histone variant H2A.Z plays an important regulatory role in various life activities of Eukaryotes.At present,the function and regulatory mechanism of Fusarium graminearum H2A.Z have not been reported.In this study,screened and the coding gene FgHtz1 of F.graminearum H2A.Z was identified through bioinformatics methods.Using the gene knockout technique,knockout mutants of the FgHtz1 gene were obtained.By analyzing the phenotype and pathogenicity of the mutants,the function of FgHtz1 gene in the growth,development and pathogenic process of F.graminearum was defined.These results laid the foundation for elucidating the molecular mechanism of FgHtzl gene regulating the growth,development and pathogenicity of F.graminearum.The main results are as follows:1.A homologous recombination vector of the F.graminearum FgHtz1 gene was constructed and transformed into the wild type strain PH-1 of F.graminearum with PEG-mediated protoplast transformation technology.Transformants were screened with glufosinate ammonium,and were identified by using PCR and qRT-PCR technology.The positive transformants were obtained and identified as knockout mutants of the FgHtz1 gene,and were named ?FgHtzl-1,AFgHtz1-2 and ?FgHtz1-3,respectively.2.The phenotype and pathogenicity of the FgHtz1 gene mutants were analyzed,and it was found that the growth rate of the mutants was slower,the pigment production was less,conidia become smaller and production decreases,and the pathogenicity was reduced to corn.These results indicated that the FgHtz1 gene was involved in the regulation of growth,development and pathogenicity of F.graminearum.3.The sensitivity of the FgHtzl gene knockout mutants to abiotic stress was detected,and it was found that the level of inhibition of the mutants was significantly lower than that of the wild type on the Congo red media,the level of inhibition of the mutants was significantly higher than that of the wild type on the sorbitol and H2O2 media.these results indicated that the FgHtz1 gene was involved in the integrity of cell membrane and in response to osmotic and oxidative stresses.
Keywords/Search Tags:Fusarium graminearum, H2A.Z, FgHtz1, mutant, pathogenicity
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