Phosphorescent Molecularly Imprinted Nanocomposites For Bioprotein Recognition Analysis

As a major player in biological function,protein has become one of the key objects of life science research.However,the composition of biological samples is complex.How to target and identify target proteins has become a bottleneck in the field of proteomics and disease diagnosis.The combination of Quantum Dots?QDs?and Molecular Imprinting Polymers?MIPs?opens up new solutions for optical identification analysis of proteins.In this study,two proteins?cytochrome c and transferrin?were selected as template molecules,and room temperature phosphorescence?RTP?Mn-ZnS QDs with good resistance to background fluorescence interference was combined with molecular imprinting technique.Two kinds of nano-composite sensors with high specificity and strong anti-interference ability were developed and successfully applied to the specific recognition and analysis of proteins in practical samples.The main contents of the study were as follows:?1?Using RTP Mn-ZnS QDs as nano-support materials and Surface molecularly imprinted polymers?SMIP?as artificial antibodies,and combining the two to prepare a selective recognition and analysis of cytochrome c?Cytochrome c,Cyt c?phosphorescent QDs-SMIP artificial antibody nanocomposites.Because this study uses the phosphorescent properties of QDs to avoid the interference of biological background fluorescence and scattered light,this method is especially suitable for trace optical recognition of Cyt c in biological fluids.In addition,Cyt c can cause a decrease in the phosphorescence intensity of QDs by electron transfer.Therefore,the method can also achieve quantitative analysis of Cyt c.The detection limit of the method is 0.015?M,and the recovery rate of Cyt c in human serum and urine is 96%to 104%.?2?Using mesoporous nano-SiO₂ as substrate and Mn-ZnS room temperature phosphorescence?RTP?QDs?PQDs?as luminescent material,a novel RTP-type protein mesoporous imprinting material?SiO₂-PQDs-MIPs?with high specificity and strong anti-interference ability was developed.And applied to target protein recognition analysis?Transferrin,Trf as an example?.The method can realize the quantitative analysis of Trf,and the detection limit of the method is 0.014?M.The recoveries of Trf in human serum and urine are 94.5%-102.0%.Since the sensing signal of the SiO₂-PQDs-MIPs on the protein is based on the phosphorescent nature of the PQDs,the interference of background fluorescence and scattered light of the complex biological sample can be avoided.Therefore,SiO₂-PQDs-MIPs are especially suitable for the specific identification and accurate quantitative detection of proteins in biological fluids.