Tetraphenylethene-based Conjugated Microporous Polymer For Aggregation-induced Electrochemiluminescence Research

This article briefly introduces the concept of electrochemical luminescence(ECL),the principle of ECL,ECL luminophore,and the applications of ECL in metal ion detection,biomolecule detection,immunoassay,aptamer sensor,and ECL imaging.Electrochemiluminescence is a highly sensitive technology with potential applications in pharmaceutical analysis,clinical diagnostics,environmental and food analysis,and immunoassay and DNA assay.ECL luminophore systems are comprised of three categories including inorganic systems,organic systems,and nanomaterial systems.In this thesis,we demonstrate the aggregation-induced electrochemiluminescence(AIECL)generated by the 1,1,2,2-tetrakis(4-bromophenyl)ethane(TBPE)-based conjugated microporous polymers(TBPE-CMPs)and its biosensing application.We synthesized three TBPE-CMPs(i.e.,TBPE-CMP-1,-2,-3)using three different molecules including tris(4-ethynylphenyl)amine(TEPA),4,4-diethynylbiphenyl(DEP)and 2,4,6-tris(4-ethynylphenyl)-1,3,5-triazine(TEPT).The TBPE-CMP-1 shows high anodic ECL emission in the presence of coreactant tri-n-propylamine(TPrA),and it exhibits high ECL efficiency(1.72%)due to the improved electron-hole recombination efficiency and efficient suppression of the nonradiative transition.In addition,the ECL property may be tuned by conjugating different ligands(e.g.,TEPA,DEP,and TEPT molecules)with TBPE to form the TBPE-CMPs which can decrease the energy gap to facilitate the injection of electron into the conjugated polymer backbone.We further demonstrate the construction of a TBPE-CMP-1-based ECL sensor for one-step detection of dopamine whose electrooxidation products may function as the energy acceptors to quench the ECL emission of TBPE-CMP-1.This ECL sensor exhibits high sensitivity and good anti-interference capability,with potential applications in biomedical research and clinical diagnosis.