The Effect Of Ectoine On DNA Condensation And Charge Neutralization

Ectoine,a cosolute and osmolyte,is used by extremophilic microorganisms to maintain an osmotic equilibrium of cells with their surrounding medium under conditions of extreme salinity or thermal and pressure stresses.It is also considered a protectant of biomolecules such as protein and DNA in cells.In the present study,we investigate its influence on DNA charge neutralization and compaction with the metal cations through dynamic light scattering(DLS),atomic force microscopy(AFM)and single molecular magnetic tweezers(MT).We found that ectoine can promote DNA charge neutralization induced by multivalent cations at mild cosolute concentration in solution.When the concentration of ectoine arrives high enough,however,a mixed effect of promotion and suppression can be found under the same ionic conditions.In this case,the electrophoretic mobility(EM)of DNA is promoted in the region of low cation concentration,while suppressed in the region of high counterionic concentration.The size of DNAis consist with the result of EM.The charge neutralization of DNAby ectoine is also related to DNA compaction.The promotion and suppression of DNA compaction by ectoine was observed by AFM imaging.The condensed structure of DNA becomes more compact and then loose once more with the increasing concentration of ectoine.Meanwhile,the condensing forces of DNA measured by magnetic tweezers shows the same trend as does the DNA EM.We explained the experimental findings through the combined effect of two intrinsic features of ectoine,preferential exclusion and enhancement of the dielectric constant of the medium.