Three-dimensional Cell Dynamic Detection Based On OCT

With the development of tissue engineering,it is becoming more and more important to visualize and track the dynamic changes of cells,scaffolds and tissues in three-dimensional structures.Optical coherence tomography(OCT)is a new type of tomography technology that has developed rapidly in recent years.Compared with traditional histological sections,OCT imaging technology can non-invasively monitor the dynamic changes of three-dimensional cells in the stent in real time.Other imaging techniques such as CT,MRI and ultrasound has low imaging accuracy,which cannot meet the needs of tissue engineering cell imaging,and CT and other rays cause irreversible damage to cells.Conventional optical imaging techniques such as laser confocal imaging have only a micron-scale imaging depth and are unable to image the three-dimensional cells in the stent as a whole.OCT technology provides high resolution(1?15 ?m)and suitable imaging depth(1?10 mm)is an ideal tool for 3D cell dynamic detection.It is of great significance to detect the dynamic changes of three-dimensional cells in tissue engineering.Using the established ultra-wideband spectral domain OCT system,the dynamic measurement of tissue thickness and the dynamic invasion of cells in collagen are quantitatively evaluated by the method of distance measurement based on scattering interface.Construct a tissue engineering skin model based on gas-liquid culture,and OCT can detect stratification of the skin and growth of keratinocytes.The growth of the skin thickness and the thickness of the stratum corneum during growth was quantitatively analyzed by using a scattering interface-based quantification method.The feasibility and accuracy of OCT for detecting tissue engineered skin was verified by comparison with H&E stained sections.In this paper,a three-dimensional tumor invasive model with a thickness of more than 1 mm was constructed.The OCT system was used to detect the cell migration and invasion dynamics,and the in vitro invasion process of tumor cells was characterized by the change of cell migration distance and the decomposition of matrix material.The peak distance of the OCT scattering interface is used to quantitatively detect the migration distance of the tumor cells,and the three-dimensional image is used to quantify the surface curvature,thickness and overall volume change of the matrix material to characterize the decomposition and deformation information of the matrix material during the invasion of the tumor cells.By designing three-dimensional tumor models with different nutritional gradients and different pH microenvironments,the OCT system was used to accurately quantify the migration distance,surface curvature,thickness and overall volume of tumor cells at different time and in vitro microenvironment.Based on OCT technology,this study can monitor the dynamic changes of three-dimensional cells in the process of growth,and quantify the growth of cells during tissue engineering and tumor cell invasion by quantitative methods based on scattering interface.The results are consistent with the gold standard.The quantitative parameters are more comprehensive and reflect the growth and mechanism of the cells.The feasibility of three-dimensional cell dynamic detection in tissue engineering by OCT has been verified by research.The research shows that OCT has a good application prospect in the application of cell dynamic changes in three-dimensional tissue model.