Discovery And Study On The Specific Response Gene And Signal Pathway Of Arabidopsis To NaHCO₃ Stress

Saline-alkali stress is a mixed stress,in which the alkaline stress caused by HCO₃-or CO₃^2-is particularly toxic to plants.However,there are few reports of bicarbonate-specific signaling pathways in plants independent of high p H and saline-alkali stress.In this study,through the stress treatment and transcriptome analysis of Arabidopsis thaliana by Na OH,NaHCO₃,Na Ac,Na2HPO4 and other alkali salts,the following conclusions were obtained:1.The pH buffering capacity of NaOH is significantly weaker than that of bicarbonate and biphosphate.2.Transcriptome analysis determined the specificity of NaHCO₃ stress: it mainly regulated the absorption and utilization of nitrogen,iron and other elements and reactive oxygen species?ROS?in roots,and mainly regulated the starvation response and energy response in leaves.By performing alkali salt sensitivity tests on mutants and overexpressing lines,the bicarbonate sensitive gene?EBS1?encoding S-ribonuclease binding protein was identified to have positive and specific regulation of plant tolerance to NaHCO₃ effect.3.The brassinolide signaling pathway regulated by NaHCO₃,Na Ac and Na2HPO4 was identified.The alkali salt sensitivity of brassinolide receptor gene BRI1 was tested.It was found that brassinosteroid signals can positively regulate the tolerance of plants to bicarbonate stress,and negatively regulate the tolerance of plants to acetate and phosphate.4.According to the analysis of transcription data,it is found that there are few p H-related pathways that are jointly regulated under the three alkali salt stresses.In addition,in the results of plant tolerance to the three alkali salts,the stress effect at high p H is not necessarily the most significant The combination of the two shows that the stress of alkali salts is mainly caused by weak acid anions.Generally speaking we identified genes that specifically respond to bicarbonate stress,found BR signaling pathway regulated by salt and alkali stress,and confirmed that the relationship between alkali stress and high p H stress is small,and mainly depends on the role of weak acid ions.These results provide more data support for the study of alkali-tolerant genes,and also provide reference for the study of phosphate and acetate.