Taxonomy Of Lebertiidae(Hydrachnidiae,Lebertioidea) In China Based On Morphology And COI

Lebertiidae Thor,1900 belongs to Lebertioidea,Hydrachnidiae,Acari.More than 450 species are known in the world.A total of 14 species,belongs to 2 subgenera,1 genus,have been reported in China from 1994 to 2019.The level of classification research lags behind that of the world,and the development of China's research area is unbalanced.There is still great potential in the study of Lebertiidae in China.It has great theoretical value to study the scientific issues such as species classification,geographical fauna and phylogeny.About 400 slides were made from Liaoning,Jilin,Heilongjiang,Fujian,Hunan,Shandong,Guangdong,Hainan and Inner Mongolia.More than 90 morphologic structures were drawn.And a total of 36 species,belongs to 6 subgenera of the genus Lebertia Neuman,1880 in the family Lebertiidae from China were described,among which 20 new species,2 new record species and 4 new record subgenera were newly identified: Mixolebertia Thor,1906;Septlebertia Imamura,1954;Eolebertia Gerecke,2009;Brentalebertia Gerecke,2008;Lebertia(Septlebertia)convergella Tuzovskij,1990;Lebertia(s.str.)ignatowi Sokolow,1930.Lebertia(s.str.)borealis Wang & Guo,2020;Lebertia(s.str.)maolanensis Wang & Guo,2020;Lebertia(Mixolebertia)xuanensis Wang & Guo,2020;Lebertia(Eolebertia)exceedia Wang & Guo,2020;Lebertia(s.str.)narrowia sp.nov.;Lebertia(s.str.)liaoningensis sp.nov.;Lebertia(s.str.)jidongensis sp.nov.;Lebertia(s.str.)hainanensis sp.nov.;Lebertia(Mixolebertia)denticleia sp.nov.;Lebertia(Mixolebertia)chishuiensis sp.nov.;Lebertia(Mixolebertia)shannxiensis sp.nov.;Lebertia(Mixolebertia)variousis sp.nov.;Lebertia(Mixolebertia)guangdongensis sp.nov.;Lebertia(Mixolebertia)fujianensis sp.nov.;Lebertia(Eolebertia)novemia sp.nov.;Lebertia(Brentalebertia)yunnanensis sp.nov.;Lebertia(Brentalebertia)wangxiaensis sp.nov.;Lebertia(Brentalebertia)neimengguensis sp.nov..The 6 subgenera of the genus Lebertia of the family Lebertiidae in China are distributed in 7 regions in 2 realms.In zoogeographic region of world,there are 5 subgenera in Palaearctic Realm,excluding Eolebertia;5 genera in Oriental Realm,excluding Septlebertia.The ratio of the number of species to the total species is from high to low: Oriental(47.22%)> Palaearctic(38.89%)> Oriental-Palaearctic pattern(12.90%).There are 12 types in zoogeographic region in China,the ratio of the number of species to the total species is from high to low: Huazhong(38.89%)> Mengxin(13.89%)> Huabei(11.11%)> Huanan(8.33%)> Dongbei = Huabei-Huazhong(5.56%)> Qingzang = Xinan = Huabei-Qingzang = Huabei-Xinan = Dongbei-Huabei-Mengxin = Dongbei-Huabei-Huazhong-Huanan(2.78%).The re-statistics of the cross-regional types with specific region,and the re-statistics of the species proportion from high to low are as follows: Huabei(16.67%)> Huazhong(8.33%)> Dongbei(5.56%)> Xinan = Huanan = Mengxin = Qingzang(2.78%).It is found that the species of acaridae were the most in Huazhong,the second in Mengxin and the third in Huabei,and Huabei is closely related to other geographical divisions.A phylogenetic tree based on the partial sequence of COI gene was constructed,with the genus Sperchon Kramer,1877 of the family Sperchontidae Thor,1900 as outgroup.It showed that the phylogenetic tree of 5 subgenera of the Lebertiidae had two clusters,one consisting of [(Lebertia s.str.)+Eolebertia+Pilolebertia+Brentalebertia],the other of monophyly Mixolebertia.The trees generated from molecular data suggested the relationship within 5 subgenera in Lebertiidae was {[(Lebertia s.str.)+Eolebertia+Pilolebertia+Brentalebertia]+ Mixolebertia}.Based on phylogenetic tree,more detailed interpretation of identification characteristics,and more clearly distinguish subgenera.