The TRPA1 Mechanism Of The Regulation Of Small Intestinal Movement By Monoamine Neurotransmitters

Abnormal intestinal function is often accompanied by the occurrence of various intestinal diseases,such as irritable bowel syndrome(IBS)and inflammatory bowel disease(IBD),while intestinal monoamine neurotransmitter secretion disorder is usually one of the causes of IBS and IBD.Transient receptor potential anchorin 1(TRPA1)is present in neurons associated with sensory pain,responds to irritating compounds in food,and plays an important role in gastrointestinal motility.The lack of TRPA1 will reduce the body’s sensitivity to environmental and chemical stimuli,and weaken the body’s perception of harmful stimuli.Therefore,studying the role of TRPA1 in the intestine has profound research significance for the treatment of gastrointestinal diseases.However,it is unclear whether the effects of different monoamine neurotransmitters on intestinal motility and whether TRPA1 can participate in the regulation of monoamine neurotransmitters on intestinal motility.In this study,the use of multi-channel physiological signal acquisition system,constant temperature perfusion system,patch clamp technology and behavioral detection methods to explore the role of monoamine neurotransmitters in the regulation of small intestine movement in mice and monoamine neurotransmitters to regulate the movement of small intestine In addition,we explored the effect of intestinal monoamine neurotransmitters on advanced central learning cognition.The main results are as follows:1.The effects of monoamine neurotransmitters on intestinal motility vary.The inhibitory effect of DA and NE on jejunal movement in mice was significantly higher than that on duodenum;the enhancement effect of 5-HT on duodenal movement was significantly higher than that on jejunum;2.TRPA1 gene knockout significantly inhibits small intestine movement in mice;3.TRPA1 is involved in the regulation of monoamine neurotransmitters on intestinal motility.After TRPA1 knockout,it weakens the inhibitory effect of DA on mouse intestinal motility,strengthens the inhibitory effect of NE on mouse intestinal motility,and enhances 5-HT Enhancement of rat duodenal movement;4.After increased intestinal DA,it damages the mice’s spatial learning and memory;After increased intestinal NE,it damages the mice’s short-term learning and memory;Increased intestinal 5-HT has no significant effect on mice spatial learning and memory;After increased intestinal monoamine neurotransmitters,the spatial long-term learning and memory of TRPA1 knockout mice are impaired.The research results show that different monoamine neurotransmitters have different regulation of the intestinal motility of the small intestine,and TRPA1 participates in the intervention of monoamine neurotransmitters on the small intestine.Behavioral testing revealed that intestinal monoamine neurotransmitter disorders can impair spatial learning and memory in mice.