Preparation Of MoO₂-Base Surface Enhanced Raman Spectroscopy Substrate And Their Application In Biological Imaging

Surface-enhanced Raman spectroscopy?SERS?as an spectrum-based detection technology,which can reflect the fingerprint information of molecules,has been developed rapidly in recent years due to its high sensitivity,non-destructive,simple and fast characteristics.The traditional SERS substrate materials were mainly noble-metals such as gold and silver,which could not fully meet people's requirements in terms of price cost and stability,and thus being limited in practical application.Although not as sensitive as noble-metals,non-precious metal SERS substrate has attracted more and more attention due to its adjustable optical properties,good biocompatibility and low cost.Therefore it is of great significance to research and develop novel non-noble metals as SERS substrate materials.In this paper,two MoO₂ nanoparticles with different morphologies were synthesized and used as SERS substrate to explore their SERS performance and mechanism,respectively.And also,they were modified as SERS probes and applied to the three-dimensional SERS imaging study of cells.Part of this paper is about the research on the medium temperature electric properties of ZrO₂ solid oxide fuel cell.Main research contents are as follows:1)The rod-assembled MoO₂ nanosphere was synthesized by solvent heat method,for which the morphology,properties,SERS performance and SERS mechanism were systematically investigated.There were a large number of nanorods on the surface of the nanomaterial,which can not only increase the surface area,but also provide a strong local surface plasmon resonance effect,thus obtaining a good SERS performance.The minimum detection concentration of rhodamine 6G?R6G?was 10^-9 M and the maximum enhancement factor was 3×10⁶.The synthesized MoO₂ SERS substrate both has good sensitivity and stability,and will not change in the acid and base environment or in the air.We also explored its SERS mechanism,both physical and chemical enhancement effects were observed in its SERS process,which played a joint role.2)The MoO₂ nano hollow spheres were synthesized by microwave heating method,and the morphology,properties and SERS performance were investigated.The hollow sphere structure can further enhance its plasma resonance effect,and thereby enhance its SERS effect.The minimum detection concentration of rhodamine 6G?R6G?was 10^-10 M and the maximum enhancement factor was 4.3×10⁷.MoO₂ hollow spheres and 4-mercaptobenzoic acid?4-MBA?molecules were assembled as probe molecules for three-dimesinal SERS imaging of cell membranes.The probe molecules can fully depict the morphology of cell membranes,providing a novel method for single cell three-dimesinal imaging.3)Basing on yttrium-stabilized zirconia?YSZ?,a variety of solid electrolytes were synthesized by solid phase and sol-gel methods.We investigated the morphology of the solid electrolyte and the electrical properties at medium temperature,and the results showed that the YSZ-MgO-NaCl/KCl composite electrolyte had the best electrical properties at medium temperature,indicating that the doping of NaCl/KCl can effectively improve the electrical properties of YSZ-MgO electrolyte.