Synthesis Of Silicon Dots With Fluorescent Properties And Their Applications In Biomolecule Detection

Silicon dots are silicon quantum dots,or silicon dots?Si dots?,and they are a type of quantum dot.Silicon quantum dots have many excellent properties,such as a wide range of sources for preparing fluorescent silicon quantum dots,low cost of preparation,low toxicity,high fluorescence,high stability,and good biocompatibility,which can achieve high sensitivity,real-time,non-destructive to biological molecules.Detection,therefore,silicon quantum dots have attracted widespread research and attention in the field of detection.In this paper,fluorescent silicon quantum dots are synthesized by aqueous phase,and different functional groups are modified on the surface of the quantum dots to give silicon quantum dots different detection performance.A fluorescent biosensor platform is constructed,and sensitive detection of glucose,hydrogen peroxide,and dopamine is successfully achieved.The main work of this article is as follows:1."All-Inclusive"Silicon Nanoparticles for Sensitive Detection of Blood GlucoseIn this study,a new type of"All-inclusive"silicon nanoparticle modified with a boric acid group as a glucose recognition unit and a PEG component with"antifouling"ability was successfully synthesized and can be used for sensitive detection of glucose in serum.The addition of glucose will cause significant fluorescence quenching of the"All-inclusive"silicon nanofluorescent probe.This method achieves sensitive detection performance for glucose in a linear range of 0.4 to 10?M,with a detection limit of 0.27?M.In addition,the method can be used for reliable clinical analysis of blood glucose by measuring blood glucose in serum.2.Metal-coordinated fluorescent silicon nanoparticles for dual-modal detection of hydrogen peroxideIn this paper,copper-doped fluorescent silicon quantum dots are hydrothermally synthesized by a one-pot method,and have maximum fluorescence under the excitation of 365 nm ultraviolet light.The synthesized Cu-Si QDs has high fluorescence intensity and HRP-like activity.In the presence of hydrogen peroxide and TMB,it can catalyze hydrogen peroxide to generate hydroxyl radicals to oxidize TMB.The oxidized TMB can absorb 365 nm excitation light and decrease the fluorescent intensity of silicon quantum dots,at the same time,the emission light At the same time,the emitted light of silicon quantum dots can also be absorbed by ox TMB via the fluorescence resonance energy transfer?FRET?effect.In this paper,we have realized dual-mode sensitive detection of H₂O₂.The linear response range of this sensing method to hydrogen peroxide by colorimetric and fluorescent methods are 1-250?M and 0.1-20?M,respectively,and the detection limits are 0.27?M and 0.068?M,respectively.3.Sensitive Detection of Dopamine by Fluorescent Silicon Nano-Ferrous IonsIn this study,silicon quantum dots were synthesized in water phase and their morphology and fluorescence characteristics were characterized.It was discovered for the first time that the synthesized silicon quantum dots can coordinate with ferrous ions.After adjusting the p H,the silicon quantum dots and ferrous ion complexes can further coordinate with dopamine to form a blood-red complex,which has a high value near480 nm.The linear absorption of the fluorescence intensity of silicon quantum dots is caused by the FRET effect.Based on the above principles,a sensitive detection of dopamine at a concentration of 0.1-20?M was achieved with a detection limit as low as 0.048?M.In short,the above-mentioned sensing method is based on the design of fluorescent silicon quantum dots,and the silicon quantum dots are endowed with different detection performance through different synthetic methods or modification methods,and the sensitive detection of glucose,hydrogen peroxide and dopamine are successfully achieved.