Tomato Defense Hormones Affected By Infection Of Bemisia Tabaci And Tomato Chlorotic Virus

Tomato chlorotic virus(ToCV)causes devastating damages to tomato and other vegetable production in China in recent years,and it is mainly transmitted by Bemisia tabaci.By controlling the signal pathway of plants,to increase the defensive compounds such as jasmonic acid(JA)and salicylic acid(SA),has been considered as a new method to control insect vectors from transmitting plant viruses,yet how JA and SA affect the transmission of ToCV by B.tabaci are still unknown.In this research,we used the techniques and methods of insect physiology,behavioral and molecular biology to study ToCV and B.tabaci Q in China which break out seriously in the field,and to focus on the impact of plant hormones after the plants have been both separately and jointly infected by ToCV and Q,and to study the metabolomics of JA and SA in plant hormones.The main results are as follows:1.By using mass spectrometry of metabolomics,three pathway maps of SA and JA metabolism on ToCV-infected tomato after 40 d have been studied.The expressions of JA and SA were all up-regulated,which indicates that the plant hormones JA and SA,were both induced on the metabolic pathway.2.We found that after the healthy Q infested on the ToCV-infected tomato after 40 d,the relative expression levels of JA and SA in plant leaves were increased compared with the control.3.We found that when the viruliferous Q infested healthy plants,the relative expression of SA in the leaves of the plants increased significantly,while the expression of JA decreased dramatically.In this research we utilized techniques and methods of metabolomics and molecular biology to study the role of JA and SA in the transmission of virus by B.tabaci.We found that the infestation of viruliferous B.tabaci promotes the inhibition of plant defense,and facilitate virus transmission.Our results will provide a theoretical basis for studies on the interaction of B.tabaci-ToCV-host plants.