Research And Development Of Imaging System Based On Micro-encoded Suspendion Array

In the field of medical diagnosis,quantitative detection of small biological molecules such as antigens and antibodies can obtain clinical diagnostic information and complete the diagnosis of diseases.As the representative of traditional biological detection technologies,enzymelinked immunosorbent assay(ELISA)and chemiluminescent immunosorbent assay(CLIA)have high maturity and stability,but at the same time,they can only complete the quantitative detection of single biological molecule in a single sample.If they are used to detect a variety of biological molecules in a single sample,the detection task can only be accomplished by increasing the detection time.Suspension chip technology provides a feasible solution to the problems faced by traditional biological detection technology.It realizes simultaneous detection of 100 biological molecules in a single sample by using fluorescence coded suspension chip technology,and has higher sensitivity and accuracy.But in this detection technology,the fluorescence coding is easily lost,which affects the stability of the detection results.In order to overcome the shortcomings of fluorescent encoding levitation chip technology in encoding,a detection system based on graphic encoding levitation chip is developed in this paper.It uses two-dimensional graphics to code 128 biomolecules at the same time.According to the principle of system design,we need to implement the following functional modules.Firstly,optical microscopic imaging equipment is used to collect bright field and fluorescence images of suspension chip.For this reason,it is necessary to realize automatic control of optical imaging equipment such as micro-mobile platform,camera and light source,and realize automatic focusing function for suspension chip.Then,the encoding information of levitation chip in open field image is identified,which includes four processes: image preprocessing,chip positioning and validation,levitation chip tilt correction and code recognition.In these four processes,several digital image processing technologies,such as image binarization,morphological operation,region filling,contour extraction,line detection,affine transformation and template matching,are used.Finally,the fluorescence signal on the surface of the suspension chip is extracted from the fluorescence image and the standard curve is fitted.On the basis of realizing the above functions,the whole system is developed.The successful development of this system will realize high throughput automatic detection of small biological molecules such as proteins and nucleic acids,and promote the further development of biomolecular detection technology in China.