Preparation Of Ledum Oil Flexible Nano-liposomes

Objective:The aims of this study were to prepare a novel flexible nano-liposomes for Ledum oil,and to optimize the preparation process.Establishing the method of content determination for Ledum oil flexible nano-liposomes and its quality evaluation.In vitro percutaneous permeation and skin irritation of Ledum oil flexible nano-liposomes were investigated.To evaluate the influence of analgesic and anti-inflammatory effects on mices,as well as flexible nano-liposomes formulations for reference.Methods:Ledum oil-loaded flexible nano-liposomes were prepared by ethanol injection.The preparation for flexible nano-liposomes were preliminary screened by single factor experiment.L9(33)orthogonal experiment was used to to optimize the process of selecting the best preparation.The ratio of Ledum oil and phospholipid(A),the ratio of Ledum oil and sodium c ho late(B),the ratio of Ledum oil and cholesterol(C),were taken as independent factors and their effect was observed on entrapment efficiency.The properties,appearance,size,Zeta potential of Ledum oil flexible nano-liposomes were characterized by transmission electron microscopy,laser particle size analyzer.The method of content determination and stability testing for Ledum oil flexible nano-liposomes was established by gas chromatography.In vitro percutaneous permeation study was performed using a TP-6 Franz diffusion cell.Investigate the irritation on normal animal skin and damaged skin by using rabbits about self contrast method.Pharmacodynamic test uses acetic acid writhing test the analgesic of Ledum oil flexible nano-liposomes,the anti-inflammatory effects was investigated using xylene-induced mouse ear swelling test.Results:The optimum preparation process of Ledum oil flexible nano-liposomes:The ratio of Ledum oil and phospholipid was 1:15,the ratio of Ledum oil and sodium cholate was1:3,the ratio of Ledum oil and cholesterol was 1:1.Ledum oil flexible nano-liposomes was milky suspension with light blue opalescent.The average entrapment efficiency was 81.6%.Its microstructure morphology was regular spherical,uniforn size.The average particle size was 143.8 nm,Zeta potential was+34.61mV.The stability test showed that Ledum oil flexible nano-liposomes in high humidity,bright light and low temperature were suitable for storage,there was white floc at high temperatures.Flexible nano-liposomes loaded Ledum oil displayed better transdermal effective compared with traditional ordinary liposomes.The rabbit skin irritation study showed that Ledum oil flexible nano-liposomes had no stimulation to the skin.Pharmacodynamic test results show that the flexible nano-liposomes loaded Ledum oil had certain effect for analgesic and anti-inflammatory.Conclusion:The preparation technology of Ledum oil flexible nano-liposomes was simple and feasible,with high entrapment efficiency.Its quality conforms to the requirements.The in vitro percutaneous permeation was better and no irritation and sensitization.Pharmacodynamic test showed that the flexible nano-liposomes loaded Ledum oil had better analgesic and anti-inflammatory effect compared with ordinary liposomes.