Study On Decomposed Recipes And Preparation Process Of Shenling Granules

Shenling Granules is composed of 9 herbal medicines,such as Rehmanniae,Codonopsis,Salvia,Scrophulariaceae,Semen,Albizia,Shichangpu,Turmeric,Poria.It is come from Bu Xin Zao Ren Tang.1.The original compositum praescriptione researchIn order to study on the status of monarch,minister,assistant and guide theory,using 80 healthy male mice,weighing20±2g,randomly divides into 8groups,such as normal group,yuanfang group,monarch group,minister group,assistant and guide group,monarch + minister group,minister+ assistant and guide group,monarch + assistant and guide group.Mice were fed the corresponding day of the test liquid in a week.Last gavage After 1h,adaptation by different compatibility groups of mice were placed in locomotor activity intervention instrument 2min,examine its impact on spontaneous activities.The results show:Bu Xin Zao Ren Tang prescription groups could decrease to varying degrees locomotor activity.Where in the original party group was reduced significantly the number of frequency of occurrence of spontaneous activity.Monarch group,monarch + minister group,,monarch +assistant and guide group significantly reduce the number of frequency of occurrence of spontaneous activity.Thus,by the demolition of modern square test confirmed the scientific prescription compatibility,further preparation for the subsequent prescription form provided.2.Research on pharmacodynamics screening processHome-made single provided oleyl alcohol sink,alone oil alcohol sink,put together oleyl alcohol precipitation and put together the oil is not alcohol extract of four groups of different process routes,using anyway,sleep reflex test examines the extracts group of mice threshold,threshold dose of pentobarbital sodium synergy effect;Four groups of extract of mouse threshold,the threshold dose of pentobarbital sodium showed different degree of synergy,especially to lift the oleyl alcohol sink group significantly shorten the sleep latency of mice(P < 0.01)and prolong the sleeping time in mice(P <0.01);Through Shenling granules four groups of different processes extract main pharmacological research and for guiding future best particle of preparation process provide experimental basis.3.Preparation technology researchDiacolation technology: with the principal components of salvia miltiorrhiza tanshinone Ⅱ A,Dan phenolic acid B transfer rate as the evaluation indexes,through the early test and orthogonal test to select the best extraction process,final and three particles diacolation process is: salvia miltiorrhiza and radix curcumae add right amount 80% ethanol for 24 h,with volume 8 times of 80% ethanol by 1 ml/min diacolation,diacolation liquid collection set aside.Volatile oil extraction technology,the main effective components in volatile oils Fang Zhongshi calamus,through test and orthogonal design test at the beginning of the best water extraction process and the inclusion process,final and three particles full calamus water extraction conditions for adding 8times amount of water,immerse 3 h,reflux extraction of 6 h.Aqueous alcohol sinking process: Israel in the mullein of radix rehmanniae and catalpa alcohol sugar transfer rate as the evaluation indexes,through the early test and orthogonal test to select the best extraction process,ultimately determine the extraction technology of water extract-alcohol precipitation and three particles as: salvia miltiorrhiza and radix curcumae after extraction technique of optimum diacolation liquid,including the best after the extraction process of volatile oil liquid,mixing prescription remaining medicinal materials in radix rehmanniae,codonopsis,radix scrophulariae,zizyphus jujube kernel,cortex albiziae,poriaVolatile oil inclusion process: to extract the volatile oil diluted with anhydrous ethanol(1:1)and beta CD under 30 ℃ after closure,according to stir 1.5h to place it in the refrigerator(4 ℃)refrigerated 24 h,the suction filter,use a small amount of distilled water and petroleum ether respectively repeatedly washing filter layer,will filter out together with the filter paper,40℃temperature drying to constant weight for a quick white powder clathrate,weighing and set aside.Enrichment process: taking mullein sugar,tanshinone Ⅱ A transfer rate as the evaluation index,by investigating the enrichment mode,temperature and concentration enrichment degree and the final diacolation liquid water extract-alcohol precipitation and the liquid combined stress concentration to the relative density was 1.2(70 ℃),the thick paste.Preparations molding process: by investigating the influence of material types used for granulating,and through batch test,molding process for the final three refs particles-dextrin starch(3:1)blend to add enrichment process under a thick paste,blending,made of soft material,18 mesh granulating drying 16 mesh granulation drying for a quick.