This paper mainly covers the following aspects:1.The importance of enantioseparation of chiral drugs and the category of chiral stationary phases were reviewed.More attention was paid to protein-based chiral stationary phases(CSPs)and the front analysis(FA)method.2.The albumin(BSA or HSA)based chiiral stationary phases were prepared successfully with 2,4,6-trichloro-1,3,5-triazine as the activator.By this method,the preparation process was relatively fast and economical.Enantioseparation of tryptophan was evaluated on the as-prepared CSPs under the reverse mode.The effects of pH value,column temperature,organic modifier on the enantioseparation of tryptophan were discussed.Chromatographic retention mechanism was studied further with the FA method.3.The ?-cyclodextrin(?-CD)bonded chiral stationary phases were synthesized using 3-glycidoxypropyl-trimethoxysilane(KH-560)as a coupling reagent,Then two composite chiral stationary phases derived from albumin(BSA or HSA)and ?-cyclodextrin were prepared using 2,4,6-trichloro-1,3,5-triazine as a cross-linker.Baseline enantioseparation of warfarin was obtained on the ?-cyclodextrin/BSA composite chiral stationary phases.Chemical transformation of warfarin was observed during the storage period.Enantioseparation of tryptophan was performed on the ?-cyclodextrin/HSA composite chiral stationary phase.The effects of pH value of mobile phase,type and content of organic modifier,and column temperature on the enantioseparation of tryptophan were investigated.The lifetime of the ?-cyclodextrin/HSA composite chiral stationary phase was studied by continuous sampling method. |