The Biomedical Application Of Prussian Blue Nanomaterials

In recent years,the tumor incidence increasing rapidly and the patient become more and more younger.The situation is very serious.Since the traditional treatment have some disadvantages need to be solved,such as high risk and side effects,people are looking for a more safe and effective treatment actively.Tumor hyperthermia,as a new and effective means for the treatment of tumor,has been the fifth largest therapy,after the surgical treatment,radiotherapy,chemotherapy and immunotherapy.It contains many kinds of treatment means,such as Magnetic Fluid Hyperthermia(MFH),and Photothermal Therapy(PTT),etc.Tumor hyperthermia,which increases the temperature of tumor area by heating,and becaues of the temperature tolerance of the normal tissue and tumor cells,it can make tumor cell apoptosis,and not damage to normal tissue,it’s advantage is much,such as no trauma,quickly,convenient,efficient and safer,etc.Up to now,it has been a hot research connect with the diagnosis.Compared to the cancer treatment,the early diagnosis of cancer,is still full of difficult and challenge.Although there are many kinds of tumor diagnosis methods,including the Computed tomography(CT),Magnetic Resonance Imaging(MRI)and Ultrasound imaging(US),Photoacoustic imaging(PAI),etc,each have their own characteristic,and if need,even can form a multimodal diagnostic system to detect whether the tumor exists or not.Now,the multi-model is mainstream patter,especially the research of diagnosis and treatment,but,the materials with good effect,excellent treatment effect,simple and fast synthesis is still quite a challenge.In this paper,we designed a very simple method to synthesis the Magnetic Prussian Blue nanoparticles(MPB NPs)for the first time at room temperature,which composite by Prussian blue(PB)and Manganese ferrite(MnFe2O4),implements the MRI T1/T2,PTT and MFH in a multi-functional platform,not only makes up for the shortage result from the single imaging,achieve the goal of accurate diagnosis,but also achieve the purpose of PTT and MFH,especially the PTT and MFH can work together,more effectively.It can realize diagnose and treatment,in the meantime,it is more convenient and efficient.In this paper,the main work includes the following several aspects:1)At first,we synthesized the uniform and stable particle of PB by the hydrothermal mothed,the size is about 100 nm,and the group of-COOH is in the particles surface,which makes the particles with negative charge.Then prepared the MnFe2O4 magnetic nanoparticles with the surface with-NH2 positively charged by coprecipitation method,and the particle size is about 20 um.And then through the method of positive and negative charges attract each other,the PB and MnFe2O4 composite together and form the MPB NPs.And through continuous characterization and analysis,finally choose the mass concentration ratio of 1:2.3(PB:MnFe2O4 = 1:2.3),the shape of particle is better in this proportion.2)and then characterized the MPB NPs by Scanning Electron Microscope(SEM),Transmission Electron Microscope(TEM),Ultraviolet and Visible spectrophotometer(UV-Vis),Vibrating Sample Magnetometer(VSM),found that the nanometer material has good water solubility,structural stability,strong Near infrared absorption capacity and good magnetic effect,and the temperature of materials can reach more than 42 ℃in both MFH and PTT experiment,with the perfect potential applications of hyperthermia treatment.And through MRI detected,the relaxation values of r1 is 5.56 mM-1s-1,have stronger T1-weighted imaging effect,and the relaxation value of r2 is 123.46 mM-1s-1,with strong T2 weighted imaging effect,showed that the material is a T1/T2 dual modal contrast agent,can be used for T1/T2 dual modal imaging.Our synthesized MPB NPs has both good T1/T2 dual modal contrast imaging(MRI)features,and good effect of heat treatment,is a good diagnostic reagents.3)Then verified the performance of the material in the animal level,the Balb/C mice was choosed and used it for MRI and hyperthermia treatment.Big change from the effect of MRI between before injection and after could been found in mice,espectially in the 1h,demonstrating that the material has good effect of T1 and T2.And then in the hyperthermia treatment,the result showed that found the group of materials + magnetic,materials + Laser,and materials+ magnetic+ Laser had good effect,the temperature of tumor area has exceeded 42 ℃ in the process of experiment,especially the group of materials+ magnetic+ Laser,the temperature was significantly increased within 5 min,the effect of hyperthermia experiment was very well,and in the follow-up treatment,the tumor size decrease,till to disappear(especially the group of materials+ magnetic+ Laser),but the other group was not.Based on the above results,the MPB NPs has good biocompatibility,T1/T2 imaging effect and excellent heat effect,showed that the material has great potential applications in the field of diagnosis and treatment.