Study On Non-enzymatic Glucose Biosensor Based On Hydroxyapatite

Glucose monitoring is of great importance in the research fields of medical diagnosis,food industry and biotechnology,etc.The biosensor for the detection of glucose,has shown many advantages,such as wide linear detection range,high sensitivity,low cost,etc.Hydroxyapatite(HAP)is an excellent bioinorganic material with good biocompatibility and chemical stability.In this work,the HAP block material with micron channel structure was used as the substrate and modified by polyaniline(PANI)to improve the ability of surface charge transfer,then the PANI/HAP composite material was prepared.Based on this,a non-enzymatic glucose biosensor electrode(PANI/HAP/Ag-Hg)was further assembled.The PANI/Pt,Ag-Hg,PANI/Ag-Hg and HAP/Ag-Hg electrodes were also prepared.The as-prepared composite materials(PANI/HAP)were characterized by means of scanning electron microscope(SEM),EDS,X-ray powder diffraction(XRD).The Cyclic voltammetry and time-current method were used to study the sensing performance of PANI/HAP/Ag-Hg for the glucose.The results indicated that the HAP(a non-conductive material)suppressed the electrochemical oxidation of glucose with Ag as the catalyst.While the conductive modification of the PANI improved the charge transfer capability.Thus the electrooxidation on the glucose was greatly enhanced by the PANI/HAP/Ag-Hg electrode.The experimental results indicated that with the concentration range from 1 to 12 mmol/L,a good linear relationship was shown between the current density and the concentration of glucose.