Investigation Of High Specificity Detection Of Triple Negative Breast Cancer Based On Magnetic/Fluorescent Responsive Upconversion Nanoprobe

It is worth mentioning that breast cancerhas become one of the main death causes in women.Therefore,it is very urgent to diagnose and treat the breast cancer.Triple negative breast cancer is a special breast cancer because of lack of its surface without Estrogen receptors?ER?and Progesterone receptor?PR?and human epidermal growth factor receptor?HER2?.It is very hazardous and easy to transfer.Because of the lack of surface corresponding receptors,it brings great difficulty to the diagnosis,treatment and prognosis.At present,the early diagnosis and detection of the triple negative breast cancer based on clinical pathology analysis is still a big challenge.The clinical pathology analysis depends on some invasive examination methods such as surgery or biopsy to get pathologic section,which has complicate operation and increases the physical pain and psychological burden to patients.The testing technology in vitro is a rapid and simple method.It can not only reduce the patient's pain,but also can provide personal diagnosis and treatment methods.The upconversion nanoparticles?UCNPs?based on the rare earth elements are with narrow size distribution and stable optical properties,particularly,the doped-rare earth compoents with much benefit that provides very high sensitivity capability in the rapid detection in many kinds of dieseases via coupling with some targeting molecules.In this thesis,the degree applicant designed a 980nm-excited Yb and Er doping UCNPs with magnetic resonance imaging?MRI?and upconversion luminescence?UCL?performance to detect triple negative breast cancer.Magnetic resonance imaging and laser confocal scanning microscope were used to observe the nanoparticles'interation with the triple negative breast cancer cells and other breast cancer ones.By analysing the changes of the fluorescence intensity of the nanoparticles incubated with different concentrations of the triple negative breast cancer cells,it was found that the detection limit in the new system was lower than 100 cells/m L.The main research work was summaried as follows:1.Fabrication and Investigation of 980nm-excited Yb and Er doped UCNPs.The fabricated UCNPs were core-shell structure with both MRI and UCL performance.The results showed that the nanoparticles were about 300nm and can be dipersed well in aqueous solution.They can exhibit excellent UCL under the excitation of 980nm laser and also show T₁-weighed MRI under 0.5T magnetic field.2.The nanoparticles linked with a peptide molecules called GE11 can specifically recognize human epidermal growth factor receptor?EGFR?high expressed triple negative breast cancer cells.By analysing the changes of the fluorescence intensity of the nanoparticles incubated with different concentrations of the triple negative breast cancer cells,it was found that the detection limitwas lower than 100 cells/mL.To sum up,a new type of multifunctional nanoprobe via a layer by layer growth method was reported.The multifunctional nanoprobe can detect out 100 cells/m L of the triple negative breast cancer in culture solution.The fabricated nanoprobes can not only diagnose the triple negative breast cancer early,but also can be extended out to other cancer types,even other more dieseases later.