Study On The Shock Resistance Of Halotolerant Microorganism Treating Mustard Tuber Wastewater

Mustard Tuber Wastewater is high in salnity,high in organic concentration,high in nitrogen concentration and phosphorus concentration,which has a strong biodegradability.It will bring series environmental pollution problems without treatment.Osmotic pressure caused by high salinity will increase inhibition of microorganisms,and the traditional biological treatment methods are difficult to be normal operated.This paper aimed to solve the shock resistance of microorganism which treatment the Mustard Tuber wastewater,based on the problems of the pickle wastewater treatment plants in Zhejiang under the pilot process.The Sequencing Batch Reactor was selected as the process of the domesticate the Activated Sludge and filter out of the halotolerant microorganism with the method of increasing salinity gradually,and the adaptability of the halotolerant microorganism was studied by analyzing the community structure of dominant bacteria and treatment effect.At the same time,sodium alginate and activated carbon were selected to embed activated sludge,which can increase the shock resistance of microorganism when the concentrate of organic and salinity changed abruptly.The conclusions were as follows.Using the Sequencing Batch Reactor can domesticate the dominant halotolerant bacteria with the salinity increasing gradually.Microscopic examination showed that paramecium,colpidium and roving bug have a good ability to adapt the high-salinity and high organic concentration of mustard tuber wastewater and water warsen.The filamentous fungus have advantages in the process of domestication without expansion,so Sequencing Batch Reactor can be used to settle the mustard tuber wastewater.The Proteobacteria(50.9%),Bacteroidetes(34.13%)and Planctomycetes(8.20%)are dominant Phylums in halotolerant microorganism.In the high-salinity environment,the Desulfomicrobium and Desulfovibrio are tested and have desulfurization function.Nitrosomonas has nitration function.Meanwhile.Paracoccus and Azoarcus are denitrifying bacteria with denitrification function.The conclusion shows that the system may simultaneously occur nitrification and denitrification under aerobic conditions.Marinobacterium and Gelidibacter have the characteristics of halotolerant,which are isolated from Marine,play an important role in degradation of organic.The best conditions of halotolerant microorganism to treat organism and ammonia nitrogen are:the concentration of organic is less than 2000 mg/L;the concentration of ammonia nitrogen is less than 60 mg/L;DO is controlled between 5 and 6;the temperature is kept between 25?30?;pH is kept between 7?8.In this condition,the halotolerant microorganism can play effectively degrade organism and ammonia nitrogen,achieving discharge standard.Sodium alginate,as a embedding carrier of activated sludge to strengthen shock resistance is feasible.The reason is that after embeding the activated sludge,microorganism will be in a relatively stable environment,which can resist the impact of the change.Meanwhile,there will form a concentration gradient when the substrate comes inside the carrier from outside.Therefore,immobilized carrier played an important role of buffer,which keeps microorganism from external shocks.