Study On Purification Of Phycocyanin By Multiple Column Chromatography

In recent years,eutrophication of Chaohu is still serious.Cyanobacteria has erupted.It is an important topic how to utilize cyanobacteria bloom.Because cyanobacteria itself contains many useful substances,such as chlorophyll,carotenoids,exo polysaccharides and algae protein.Extracting phycoprotein from cyanobacteria,especially phycocyanin and phycocyanin,is a hot topic,because many studies have shown that phycocyanin and phycocyanin are antioxidant,anti-inflammatory,anticancer,antiaging,bacteriostasis,immunity,immunofluorescence and other physiological activities,and are widely used in food and cosmetics.Medicine and other fields.Therefore,in this paper,cyanobacteria in Chaohu was used as the material.First,the algal blue liquid was extracted by optimizing the two step salting out method,and then the high purity phycogallinin was obtained by optimizing the column chromatography.The main contents of the study are as follows:1.,two step salting out combined with gel chromatography to extract and purify phycocyanin.The types of gel column chromatography,the type of eluent,the pH of eluent and the flow rate were investigated.The optimum conditions were as follows: the eluate was phosphate eluant,the pH of eluent was 7 and the flow rate was 1.5cm/min.2.,two step salting out combined with ion exchange chromatography to extract and purify phycocyanin.The types of ion exchange column chromatography,the pH,the concentration of the eluate,the rate of addition and the concentration of salt in the eluent were investigated.The optimum conditions were as follows: the selection of filler was cellulose A-500,the eluent pH was 7,the concentration was 1-2mg/L,the adding rate was 2cm/min and the NaCl concentration of the eluent.For 0.25mol/L.3.,two step salting out and affinity chromatography were used to extract and purify phycocyanin and phycocyanin.According to the type of affinity column chromatography,the pH,the concentration of the eluate,the concentration of the sample,the concentration of the sample,the concentration of the eluant,the optimum conditions are as follows: the selected packing is hydroxyapatite,the eluent pH is 6.5,the concentration is1.6-2.0mg/L,the addition rate is 2cm/min and the PBS concentration of the eluent is 40mmol/L And 60mol/L.