Protein Denaturation And Fibrosis Monitoring And Their Biomaterial Application

The interaction between molecules and proteins has been used in various research directions and can be used to achieve many research purposes.In this paper,this special force is mainly used to screen amyloid fiber probes and construct new fluorescent nanoparticles.Amyloidogenic agglomeration caused by misfolding of proteins is a key factor in inducing many related diseases.The development and research of novel small molecules with the monitoring function and inhibitory potency of amyloid formation process are urgently needed.In this study,because of the interaction between small molecules and denatured proteins,we studied the ability of a traditional dye small molecule,bromophenol blue(BPB),to accurately recognize amyloid and effectively hinder Amyloid formation.Bovine insulin was selected as the amyloid model.During the process of insulin fiber,three stages of insulin fiber were successfully reduced using BPB as a small molecule for monitoring.When BPB was added as an inhibitor to the insulin system,the progress of fibrosis was significantly slowed,and the total amount of amyloid was greatly reduced,even complete inhibition.TBNCs nanoparticle is a new type of water-soluble nanoparticle with good biocompatibility designed and constructed by us.Heated bovine serum albumin serves as the skeleton of the nanoparticles.Since thioflavine T has been commonly used as a dye for amyloid fibers,it can specifically bind to amyloid fibers.Therefore,in the process of heating bovine serum albumin,ThT is doped in the framework of bovine serum albumin.Since the denaturation of bovine serum albumin produces a structure similar to amyloid fibrils,ThT binds to denatured bovine serum albumin to construct nanoparticles of TBNCs.The simple process,low cost,environmental protection,good biocompatibility,strong fluorescence properties make the nanoparticle worthy of popularization and application.In addition,TBNCs can label DNA.Especially as a nucleolous dye,this nanoparticle is unique.In the article,the nanoparticles have been successfully used to mark the nucleolus of HeLa cells.