Controllable Synthesis Of Nano Metal Sulfide(Oxide) And Their Cellular Biological Effects

In recent decades,with the development of nanotechnology,nanomaterials have become the frontier of research in many fields such as chemistry,materials,and medicine.For example,in the medical field,nanomaterials have been used in fields such as drug delivery,bioimaging,and photothermal therapy.As an important part of nanomaterials,nanometal sulfides or oxygen compounds have attracted the attention of researchers due to their excellent physical and chemical properties.The research shows that due to the diversity of structure,good performance,and wide application of nano-metal sulfides or oxygen compounds,people have great interest in research.However how simple and controllable synthesis of nanometer metal sulfide and oxygen compounds with special morphology,studies its formation process,as well as the research of the relationship between the morphology and properties,have become the forefront of current chemical research today.In this paper,we used the gas diffusion method and urea homogeneous precipitation method to control the synthesis of two different crystalline silver sulfide nanoparticles and three different morphologies La₂O₃ nano hierarchical structures by simply adjusting the reactant concentration,reaction time,stirring speed,or reaction temperature.And its formation process and cell biological effects were investigated.At the same time,the mechanism of its cell biological effects was studied.?1?The related knowledge of nano-metal oxygen compounds,the research achievements and latest developments of nano-metal sulfur?oxygen?compounds are briefly described.The main problems and the solutions in the current research of nano-metal sulfur?oxygen?compounds are summarized.We've outlined the topic background,research content and innovation of this paper..?2?Using the gas diffusion method,the crystalline Ag₂S nanoparticles were controllably synthesized and their biological activity was investigated.The products were characterized by XRD,EDS,HRTEM,Zeta potential,TG-DSC,FT-IR,UV-Vis and PL.The results showed that the crystalline Ag₂S nanoparticles were successfully synthesized.The average particle size is 8.97nm.The surface potential measured after dispersion in secondary water is-34.3mV,indicating that the sample has good stability in secondary water and has good optical properties.Further tests showed that the crystalline Ag₂S nanoparticles have good cell compatibility and show good PDT activity under light conditions.The mechanism of the photodynamic?PDT?activity of the sample was investigated by flow cytometry?FCM?and laser confocal?CLSM?.Studies have shown that the crystalline Ag₂S nanoparticles show good performance on human lymphoma?Raji?cells.The PDT activity will not cause cancer cell necrosis and apoptosis,but it will inhibit the growth and reproduction of cancer cells,thereby inhibiting cancer cells;through reactive oxygen species?ROS?,mitochondrial membrane potential?JC-1?and energy metabolism.?OCR?tests have further studied the mechanism,and the test results show that the PDT activity of Ag₂S nanoparticles is due to the production of a large amount of reactive oxygen species?ROS?,resulting in oxidative damage and mitochondrial dysfunction.Affects the energy metabolism?OCR?of cancer cells.?3?Based on the gas diffusion synthesis method,two different crystalline silver sulfide nanoparticles,namely amorphous silver sulfide nanoparticles?A-Ag₂SNPs?and crystalline silver sulfide nanoparticles?C-Ag₂SNPs?,are controllably synthesized by simply controlling the reactant concentration,reaction time,stirring speed,or reaction temperature.Their physicochemical properties and cell biological effects were explored.The results of XRD,EDS,HRTEM,Zeta potential,TG-DSC,FT-IR,UV-Vis and PL show that the amorphous Ag₂S and crystalline Ag₂S nanoparticles were successfully synthesized with average particle sizes of 5.69nm and 8.97nm,respectively.surface potentials dispersed in the secondary water test were-28.7mV and-34.3mV,respectively,which indicat that the sample has good stability in the secondary water.The cytotoxicity?MTT?test showed that the silver sulfide nanoparticles exhibited anti-proliferative effect on the crystal form of cancer cells.The amorphous silver sulfide nanoparticles can specifically inhibit the proliferation of cancer cells,while the crystalline silver sulfide nanoparticles Particles do not have this property.The mechanism of action was investigated by flow cytometry?FCM?,laser confocal?CLSM?and inductively coupled plasma?ICP-MS?tests.The results showed that compared with crystalline Ag₂S nanoparticles,amorphous Ag₂S nanoparticle can cause cell necrosis and late apoptosis.It has significant cytotoxicity to cancer cells and can significantly inhibit the proliferation of cancer cells.The difference in cell inhibition between two different crystalline Ag₂S nanoparticles has nothing to do with the internalization efficiency of the sample,but is related to the nature of the sample itself.The mechanism was further studied using reactive oxygen species?ROS?and mitochondrial membrane potential?JC-1?tests.The results showed that the silver sulfide nanoparticles exhibited anti-proliferative effects on cancer cells in a crystal-dependent manner.The reason is that the amorphous Ag₂S nanoparticles induce the increase of endogenous reactive oxygen species?ROS?in cancer cells and directly affect the cell mitochondria,which cause oxidative damage and mitochondrial dysfunction.This leads to cell apoptosis and necrosis.?4?A series of different morphology La₂O₃ nano hierarchical structures were synthesized by simple urea homogeneous precipitation method,And the formation process and cell biological properties were investigated.The XRD,XPS,HRTEM,FESEM UV-Vis and PL test results show that three different morphologies La₂O₃ nano hierarchical structures have been successfully synthesized.In the course of synthesizing samples,the samples at different times were characterized by HRTEM and FESEM,and the formation process was followed.Finally,the cytobiological properties of three different morphologies La₂O₃ nano hierarchical structures were characterized by cytotoxicity?MTT?experiments.?5?The research results were analyzed,summarized and summarized.Using gas diffusion method and urea homogeneous precipitation method,two different crystal forms of silver sulfide nanoparticles and three different morphologies La₂O₃ nano hierarchical structures were controllably synthesized by simply controlling the reaction conditions.And its formation process and cell biological effects were explored.At the same time,the mechanism of its cell biological effects was studied.