The Applications Of CuInZnS Quantum Dot-based Fluorescence And Electrochemiluminescence Nanosensors

CuInZnS quantum dots(QDs),as a kind of novel quaternary,not only own the unique optical properties of the traditional QDs,but also have the advantage of no toxicity of heavy metal elements such as Cd,Hg,Pb.In addition,Cu InZnS QDs are of good biocompatibility and of tunable fluorescence color.As a result,the CuInZnS QDs have aroused intensive attention in chemical,optical,medicine and electronics and the other field.In this article,we have involved the hydrothermal synthesis method to prepare CuIn ZnS QDs,a novel aptamer fluorescence nanosensor for adenosine detection based on Cu InZnS QDs and highly sensitive electrochemiluminescence detection of EGFR gene based on CuIn ZnS QDs.In chapter 1,we mainly introduced three parts,the optical property of QDs,the recent research about synthesis methods of CuIn ZnS QDs,and analytical applications of QDs.In chapter 2,we developed an aqueous synthetic strategy for a new kind of hydrophilic multicolor Cu InZnS QDs.The synthesis conditions of high quality CuInZnS QDs with tunable fluorescence color,including precursor ratio,reaction time and temperature,have been studied deeply.In addition,Cu In ZnS QDs were characterized by all kind of common methods.In chapter 3,we described a novel aptamer-based fluorescence assay for adenosine using CuIn ZnS QDs.Through the specific recognition reactions between the aptamer and analyte,the adenosine was determined selectively by the changed signal.In chapter 4,we used CuIn ZnS QDs as a novel kind of ECL luminophore to detect EGFR Gene.To enhance the sensing system sensitivity,the detecting platform was built based on the Surface Plasmon Resonance(SPR)effect between Au NPs and CuInZnS QD_S.In addition,the effect of the capped stabilizer on the ECL signal of QDs was further investigated.