Sonochemical Preparation Of Folate-targeted Reductive-responsive Nanocapsules

In recent years,microcapsules have gained wide attentions in the field of biomedical as a novel drug carrier due to its numerous advantages,such as good biocompatibity,high drug loading capacity.With the continuous development of the research of microencapsulation technique,researchers have made further efforts to give microcapsules new properties,for instance,targeting ability,stimulus responsiveness.In addition,there are lots of methods to prepare microcapsules,such as layer-by-layer self-assembling,solvent evaporation method,microemulsion technique,compared to the approaches mentioned above,the sonochemistry method has been proved to be a facile,efficient and cost effective method.In this paper,in the case of ultrasonic radiation,two kinds of FA functionalized reductive-responsive nanocapsules have been fabricated using FA,?-Poly-L-lysine,starch and so on,besides,the targeted ability and controlled release function of nanocapsules has also been investigated.1.A novel,compatible FA functionalized redox-responsive ?-PL nanocapsules(FA-?-PLNCs)were fabricated by the high intensity ultrasonic on the interface between the soybean oil and FA functionalized thiolated ?-PL aqueous solution.Coumarin 6(C6,a model of hydrophobic drugs)were loaded into the nanocapsules by dissolving in the soybean oil before sonication,the-SH could be crosslinked by sonication and formed disulfide bonds.The experimental results shows that the obtained FA-?-PLNCs which possess excellent reduction responsiveness and the FA-receptor-mediated targeting ability would be a promising drug carrier for targeted delivery and controlled release of hydrophobic drugs in the field of biomedical applications.2.The folic acid functionalized reductive-responsive starch-based nanocapsules(FA-RSNCs)have been synthesized by sonochemical method.The targeted molecule FA was immobilized onto the nanocapsule wall,and a green hydrophobic fluorescent dye Coumarin 6(C6),used as a substitute of hydrophobic drugs,was encapsulated into the NCs.The following analysis shows that,the as-prepared FA-RSNCs showeda good selectivity to cancer cells,and a perfect reduction-triggered drug release property.The size,biocompatibility and biodegradability of FA-RSNCs satisfied the demands of blood flowing.Therefore,the synthesized FA-RSNCs could be perfect intellective drug carriers for targeted delivery and controlled release of tumor drugs for the cancer therapy.