Research On The Efficiency And Regulation Of Partial Nitrification/anammox Process For Treatment Of Phenol- And Ammonia-containing Wastewater

In this paper,the operational efficiency and regulation of Sharon-Anammox and Single-stage partial nitritation/Anammox process treating phenol-and ammonia-containing wastewater were studied.The ratio of phenol to nitrogen,AOB activity,NOB activity,SAA,heterogeneous nitrification activity,denitrification activity,substrate utilization rate?SUR?and specific oxygen uptake rate?SOUR?were measured to obtain the treatment efficiency and regulatory mechanism of the reactor.In addition,the evolution mechanism was revealed by analyzing the changes of community structure during the treatment of phenol-and ammonia-containing wastewater by Sharon-Anammox and Single-stage partial nitritation/Anammox process.The following results were obtained:?1?In the Sharon-Anammox process,membrane separation technology was used to assure the concentration of AOB and avoid the competition between microorganisms.The results showed that the partial nitrification reactor was successfully started and operated stably in only 27 days,and the total nitrogen removal efficiency of the Sharon-Anammox process was as high as 82.4%.The experimental results showed that the tolerance of anaerobic ammonia-oxidizing bacteria to phenol was stronger than that of AOB and NOB.This indicated that anammox process was suitable for the treatment of phenol-and ammonia-containing wastewater.In addition,the higher ratio of phenol to nitrogen could completely inhibit the activity of AOB and NOB.The AOB activity could be recovered in time by reducing the ratio of phenol to nitrogen?m/m?.?2?After inoculated anaerobic ammonia-oxidizing sludge,the total nitrogen and COD removal efficiency could reach 73%and 79.5%,respectively,during the Single-stage partial nitritation/Anammox process in 26 days.Anaerobic ammonia oxidizing bacteria would decay in the initial stage of reactor operation,the corresponding SAA values decreased from 28.85±0.97 mg NH₄⁺-N·g^-1VSS·d^-11 to19.30±2.44 mg NH₄⁺-N·g^-1VSS·d^-1.However,the SAA values would recover(increased to 20.85±1.01 mg NH₄⁺-N·g^-1 VSS·d^-1)during the stable period.?3?When the ratio of phenol to nitrogen was 0.5,the dominant microbial communities in partial nitrification reactor were Pseudofulvimonas?14.66%?,Gemmatimonas?14.57%?,Nitrosomonas?9.99%?,Aquimonas?6.14%?,Zoogloea?3.77%?,Thauera?3.23%?,and Truepera?2.69%?.The sludge was to degrade phenol and oxidize partially NH₄⁺-N.However,when the ratio increased to 1.5,the Nitrosomonas were severely inhibited and the AOB activity dropped to zero.When the concentrations of phenol and ammonia nitrogen were 300 mg·L^-1 and 600 mg·L^-1,respectively,the activity of heterotrophic nitrifying bacteria was inhibited,and the abundance of Nitrosomonas increased and partial nitrification process was recovered immediately.Nitrosomonas continued to grow and maintain its dominant abundance during the stable period of Single-stage partial nitritation/Anammox process,while Nitrospira,a nitrite oxidizing bacteria,was almost completely inhibited.Finally,it was found that Candidatus Brocadia and Candidatus Kuenenia were dominant bacteria for treating phenol-and ammonia-containing wastewater during the stable treatment period of the Single-stage partial nitritation/Anammox process.