| Microalgae have been comprehensively widely applied in food industry,animal feed,environmental monitoring,cleaning up the environment,biological technology,renewable energy and other fields.However,the high cost of algae harvest process has been the bottleneck for scale-up application due to the small size of microalgae.Therefore,reducing the cost of separation and harvest,and improving the rate of recovery are the key point to the microalgae's commercial application.Biological microalgae harvesting method,especially fungi-based microalgae bio-flocculation method,is considered as one of promising methods due to its advantage of safety,high harvesting rate,low energy input,low water content,high biomass and lipid productivity and media recycling capability.In this study,we construct a fungal-assisted algal immobilized system by using the filamentous fungi within two various methods,and the influence factors and mechanism has been discussed.Moreover,the wastewater treatment efficiently of algae-fungi pellets was also explored.Research results were included as follow:(1)Fungus Penicillium with fast growth,well pelletization ability and strong adaptability was successfully separated from sewage and formed pellet well.(2)Optimization the process of co-cultivation of microalgae and filamentous fungus for microalgae harvest.The optimum condition was 10 g/L glucose concentration,40?,initial spore inoculum of 10~3 cells/ml,initial medium optimum pH of 5.(3)Optimization the process of fungi-pellets based microalgae harvest method.The optimum condition was fungi/algae=1:2(DW),34?,160 rpm,initial medium optimum pH of 4.(4)Fungi-microalgae pellets have a high tolerance with synthetic wastewater by synergistic action of fungi and microalgae.(5)Scanning electron microscopy results showed that fungi-algae pellets were grow well in synthetic swine wastewater.Mycelium is covered with microalgae cells,and there is an exchange of material and energy between fungi and algae. |
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