Study Of Spectral Analysis Based On Gold/Silver Nanomaterials To Detect The Residues Of Organic Pesticide

In this paper,gold/silver nanomaterials were used to construct bioenzyme sensors.Organophosphorus pesticides(OPs)were analyzed by colorimetric or fluorescence.The method of detecting organophosphorus pesticides has the advantages of high sensitivity and selectivity,simple operation,short time and can be used effectively in real samples.The main contents of this paper are as follows:Chapter one: It describles the effects of organophosphorus pesticides on the environment and some properties of gold/silver nanomaterials and the importance of acetylcholinesterase on the human body.It is briefly described the content of this paper.It also describles the colorimetric and fluorescence used in the fileld of analyze.Chapter two: A color-sensing,rhodamine B/gold nanoparticle(RB-AuNPs)combined with 3D printing technology has been designed with a self-propelled micromotor(SPM)for underwater detection of organophosphorus and carbamate pesticides.According to the color change of RB-AuNPs on SPM,the degree of river water pollution is judged.Chapter three: A highly sensitive sensor for determination of carbamate pesticides-based rhodamine B(RB)modified silver nanoparticle(RB-AgNPs)was developed.This method can simultaneously detect the carbamate pesticides by dual-reading using UV and fluorescence spectra.The morphology and chemical properties of RB-AgNPs were analyzed by transmission electron microscopy,ultraviolet-visible spectroscopy and fluorescence spectroscopy.The color of RB-AgNPs and the change in fluorescence of Rhodamine B were linearly related to the concentrations of carbamate pesticides.Chapter four: Due to the specific structure of OPs,OPs can be directly detected by photoresponsive ultrasensitive inorganic/organic nanohybrid fluorescent probes(RB-Ag/AuNPs).The method has a short response time and can be applied to real samples.Chapter five: A highly sensitive fluorescent probe based on bovine serum protein-protected gold nanoclusters(Au NCs)was developed for the determination of organophosphorus pesticides(OPs).At first,BSA-AuNCs were prepared using a photo stable fluorescent substrate.Acetylcholinesterase(AChE)catalyzes the hydrolysis of acetylthiocholine iodide(ATCI)to produce thiocholine(TCh),and the sulfhydryl groups(SH–)on TCh form Au–S bonds with BSA-AuNCs;thus,the fluorescence of AuNCs weakens.On the other hand,OPs can inhibit the activity of AChE,thus preventing the generation of TCh,and the fluorescence recovery of BSA-AuNCs occurs.The assay demonstrated highly sensitive applications in the quantitative determination of OPs in water and food samples.The innovations in this study are:(1)Combining nanomaterials with 3D printing technology,a colorimetric sensor has been prepared for the detection of pesticide residues in waters with good sensitivity and selectivity;(2)Fluorescence spectroscopy has been successfully applied to the detection of pesticides with several well-performing sensors.